固相微萃取/气-质联用法分析香连制剂中挥发性成分

目的： 利用固相微萃取/气相色谱-质谱联用法（SPME/GC-MS）分析香连制剂（水丸/浓缩丸/片/胶囊）中的挥发性成分。方法： 采用SPME法提取挥发性成分，GC-MS法对挥发性成分进行分离鉴定，建立其指纹图谱，并对香连制剂样品进行相似度评价和主成分分析。结果： 对24批不同剂型香连制剂进行分析，确定了15个共有峰，构成了香连制剂挥发性成分的指纹图谱，其中6个共有峰得到了确认，分别为去氢木香内酯，β-榄香烯，β-石竹烯，氧化石竹烯，α-紫罗兰酮和β-紫罗兰酮。结论： 水丸、浓缩丸与片剂、胶囊中挥发性成分组成相似，但在相对含量上存在差异。

Analysis of volatile compounds in Xianglian preparations by SPME/GC-MS

OBJECTIVE To analyze volatile compounds of Xianglian (XL) preparations by solid phase microextraction/gas chromatography-mass spectrometry (SPME/GC-MS).METHODS Volatile compounds were extracted by SPME and then analyzed by GC-MS.Moreover,XL samples were further evaluated by similarity evaluation and PCA analysis.RESULTS Fifth common peaks were determined in GC-MS fingerprints of volatile constituents from 24 batches of XL preparations.Among them,six peaks were undoubtedly identified,which were dehydrocostus lactone,β-elemene,β-caryophyllene,caryophyllene oxide,α-Ionone and β-ionone.CONCLUSION Composition of volatile compounds in watered pills and condensed pills are similar to those in tablets and capsules,but relative contents of volatile compounds were quite different among them.