维甲酸作用下胎鼠腭突发育期间母体的血浆代谢组学分析

目的:建立维甲酸诱导C57BL/6J小鼠腭裂模型,利用核磁共振技术和主成分分析方法分析胎鼠腭突发育期间孕鼠血浆的特征性代谢变化。方法:将孕鼠随机分为维甲酸实验组和植物油对照组,分别在ED12.0予孕鼠以维甲酸和植物油灌胃,在特定孕期收集孕鼠血浆样本和统计胎鼠腭裂发生率。应用核磁共振波谱仪测定一氢核磁共振波谱图和主成分分析法(PCA)描绘胎鼠腭突发育期间维甲酸作用下C57BL/6J孕鼠血浆代谢产物的变化轨迹图。结果:PCA分析结果显示腭发育期间两组孕鼠血浆代谢轨迹不同。结论:维甲酸可以延缓孕鼠胚胎发育期间血浆的代谢轨迹,说明母体环境变化与胚胎发育两者之间存在关联性。

Metabonomic Approach to Analyze the Pregnant Mice Plasma during Embryonic Palate Development under the Influence of Retinoic Acid.

Objective: To analyze the changes of pregnant mice plasma during the embryonic palate development using nuclear magnetic resonance (NMR) technology and the principal component analysis (PCA) method through the establishment of retinoic acid induced cleft palate in C57BL/6J mice model. Methods: Pregnant mice were randomly divided into experimental group (retinoic acid) and control group (vegetable oil), during specific pregnancy with lavage of retinoic acid and vegetable oils. After pregnant mice were sacrificed, plasma samples were collected in specific pregnancy and the incidence of mice cleft palate was assessed. Nuclear magnetic resonance spectrometer was used to determine the nuclear magnetic resonance hydrogen spectrum diagram combined with PCA, and then the track chart of plasma metabolites changes was plotted in depict retinoic acid induced C57BL/6J pregnant mice. Results: PCA analysis showed that the track chart of plasma metabolites changes between two groups was different. Conclusion: Retinoic acid can delay the metabolism of pregnant mice plasma during embryonic development, illustrating the correlation between maternal environmental changes and embryonic development.