白英总皂苷的抗炎活性研究

目的:观察白英总皂苷的抗炎活性。方法:培养人脐静脉内皮细胞(HUVEC),用200 μmol·L^-1H₂O₂损伤细胞;培养小鼠单核/巨噬细胞(RAW 264.7),用脂多糖(LPS)诱导;抽签法随机分成正常组、模型组、阳性对照组、白英总皂苷低、中、高剂量组。给药后,继续培养,应用CCK-8法观察细胞存活率;饲养SD大鼠,抽签法随机分为正常组、模型组、阳性对照组、白英总皂苷低、中、高剂量组,观察白英总皂苷对角叉菜胶诱导大鼠急性踝关节肿胀度的影响,测定前列腺素E₂(PGE₂)、环氧化物水解酶-2(COX-2)含量。结果:白英总皂苷中剂量对H₂O₂损伤的HUVEC细胞的影响以及高剂量组对LPS诱导的巨噬细胞RAW 264.7的影响,均和阳性对照组相当,无统计学差异(P>0.05);中、高剂量白英总皂苷能够明显降低大鼠急性踝关节肿胀度,降低大鼠足趾渗出液中PGE₂、血清中COX-2含量,且和模型组差别有统计学意义(P<0.01)。结论:白英总皂苷具有明显的抗炎作用,有必要进一步深入研究其药效及作用机制。

Anti-inflammatory activity of total saponins from Solanum lyratum Thunb

OBJECTIVE To observe anti-inflammatory activity of lyratum thunb total saponins.METHODS HUVEC cells were cultivated and damaged with 200 μmol·L^-1 H₂O₂. RAW 264.7 cells were cultivated and induced by LPS. Cells were randomly divided into normal group, model group, positive control group and lyratum thunb total saponins group (low, medium and high doses) by lottery. Cells were continuously cultivated after medication. CCK-8 method was applied to observe cell survival. SD rats were fed up and randomly divided into normal group, model group, positive control group, lyratum thunb total saponins group (low, medium and high doses) by lottery. Effects of lyratum thunb total saponins on acute ankle swelling in rats induced by carrageen glue, and contents of PGE₂ and COX-2 were determined.RESULTS Effects on HUVEC damage by H₂O₂ in lyratum thunb total saponins medium dose group, and effects on RAW 264.7 induced by LPS in lyratum thunb total saponins high dose group, were consistent with those in positive control group (P>0.05). Medium and high concentrations of lyratum thunb total saponins significantly reduced rat acute ankle swelling degree, PGE₂ in rat toe exudate and cox-2 content in serum, significantly different from those in model group (P<0.01).CONCLUSION Lyratum thunb total saponins have obvious anti-inflammatory functions. It is necessary to further investigate efficacy and mechanism of action.