丹参地上部分SB-4-5075-0部位对大鼠肾主动脉的舒张作用

目的： 观察丹参地上部分SB-4-5075-0部位对给予不同预收缩刺激的大鼠肾主动脉的舒张作用。方法： 采用DMT Myograph肌动描计系统记录离体血管张力。观察SB-4-5075-0 部位（10^-5～1 g·L^-1）对基础状态和用K⁺（60 mmol·L^-1）、PE（10^-5 mol·L^-1）、5-HT（10^-5 mol·L^-1）分别预收缩的内皮完整血管的舒张作用；观察SB-4-5075-0 部位（10^-5～1 g·L^-1）对用K⁺（60 mmol·L^-1）、PE（10^-5 mol·L^-1）、5-HT（10^-5 mol·L^-1）分别预收缩的去内皮血管环的舒张作用。高效液相检测SB-5075-0中主要有效成分。结果： 在内皮完整的血管上，SB-4-5075-0部位对基础状态的血管没有舒张作用；在不同物质刺激预收缩的内皮完整及去内皮的血管上，SB-4-5075-0部位在（10^-2～1 g·L^-1）剂量区间范围内对用K⁺和5-HT预收缩的血管有显著舒张作用（P<0.05，P<0.01），对用PE预收缩的血管没有明显的舒张作用；SB-4-5075-0部位的主要有效成分为丹参素、原儿茶醛和咖啡酸。结论： SB-4-5075-0部位对正常血管无舒张作用，对K⁺和5-HT引起的血管收缩均有良好的舒张作用。

Vasodilatory effects of SB-4-5075-0 fractions of Salvia miltiorrhiza aerial parts on rat renal artery

OBJECTIVE To investigate vasodilatory effects of SB-4-5075-0 fractions of Salvia miltiorrhiza aerial parts on ex-vivo rat renal artery.METHODS By using DMT Myograph in vitro vascular tension meter system,muscular responses of rat renal arterial ring by SB-4-5075-0 fractions were investigated.Diastolic function of intact or denuded endothelial rat renal artery were observed on basic state and stimulating state by K⁺ (60 mmol·L^-1),PE (10^-5 mol·L^-1) and 5-HT (10^-5 mol·L^-1).HPLC was applied to determine contents of SB-4-5075-0 fractions of Salvia miltiorrhiza aerial parts,by using Diamonsil C₁₈ column.RESULTS Within 10^-5-1 g·L^-1 dose range,SB-4-5075-0 fractions showed no relaxing effects on basic state artery.Within 10^-2-1 g·L^-1 dose range,artery was relaxed after stimulating by K⁺ or 5-HT (P<0.05,P<0.01).Within 10^-2-1 g·L^-1 dose range,artery was not relaxed after stimulating by PE.Main components in SB-4-5075-0 fractions were tanshinol,protocatechuic aldehyde and coffeic acid.CONCLUSION SB-4-5075-0 fractions can relax artery stimulated by K⁺ or 5-HT.