牙龈干细胞对实验性自身免疫性脑脊髓炎的治疗作用

目的:研究牙龈干细胞(Mesenchymal stem cells derived from gingiva, GMSCs)对小鼠实验性自身免疫性脑脊髓炎 (experimental autoimmune encephalomyelitis, EAE) 的治疗作用,为干细胞治疗自身免疫性脑脊髓炎提供实验依据。方法:通过GMSCs与T细胞体外共培养,研究GMSCs对T细胞凋亡、增殖以及向Treg细胞和Th17细胞分化的影响。建立小鼠EAE模型,研究GMSCs对EAE的治疗作用。结果:GMSCs在体外共培养中诱导T细胞凋亡, 同时抑制T细胞增殖。GMSCs可以促进Treg细胞分化,而抑制Th17细胞分化。GMSCs注射对小鼠EAE具有治疗作用。机制上GMSCs注射促进EAE小鼠中Treg细胞分化,抑制Th17细胞分化,抑制炎症反应。结论:GMSCs通过促进Treg细胞分化,抑制Th17细胞分化对小鼠EAE具有治疗作用。

Therapeutic Effects of Mesenchymal Stem Cells Derived from Gingiva on Experimental Autoimmune Encephalomyelitis.

Objective: To analyze the therapeutic effects of mesenchymal stem cells derived from gingiva (GMSCs) on experimental autoimmune encephalomyelitis (EAE) and to provide experimental evidence for treatment of EAE using stem cells. Methods: GMSCs and T cells co-culture system were established to analyze the role of GMSCs on T cell proliferation, apoptosis, Th17 and Treg cells differentiation. EAE model was established in mice to explore the therapeutic effects of GMSCs on EAE and the underlying mechanisms. Results: In GMSCs and T cells co-culture system, GMSCs induced T cells apoptosis and inhibit T cells proliferation. GMSCs also inhibited Th17 cells differentiation and promoted Treg cells differentiation. In vivo, GMSCs could ameliorate EAE in mice. Mechanically, GMSCs infusion increased Treg cells differentiation and inhibited Th17 cells differentiation. Conclusion: GMSCs infusion ameliorated EAE in mice by up-regulating Treg cells differentiation and down-regulating Th17 cells differentiation.