鼻阻塞对幼年大鼠髁突骨髓间充质干细胞成软骨分化的作用

目的 通过幼年大鼠开口呼吸动物模型探讨鼻阻塞对髁突骨髓间充质干细胞（BMSCs）成软骨向分化的作用。方法 40只4周龄SD大鼠随机分为2组,双侧鼻阻塞组28只,阻塞时间为8：00-16：00,至8周;对照组12只,不作处理。在建模初始、2周末、4周末分别取2组双侧髁突BMSCs体外培养并鉴定,CCK-8法比较2组细胞的增殖差异,RT-PCR法检测2组BMSCs成软骨标志基因（Agg、COL-Ⅱ、SOX-9）表达的差异。采用 SPSS19.0软件包对实验数据进行统计学分析。结果 鼻阻塞组髁突BMSCs的细胞增殖曲线与对照组无显著差异（P>0.05）,但成软骨标志基因的表达显著下降（P<0.05）。结论 幼年大鼠双侧间歇性鼻阻塞致其生长发育期开口呼吸,出现下颌骨发育不足,与髁突BMSCs软骨向分化受到抑制有关。

Effect of condylar bone marrow-derived mesenchymal stem cells on chondrogenic differentiation in young rats induced by nasal obstruction

PURPOSE: This study was designed to utilize the animal model of nasal obstruction to investigate its effect on chondrogenic differentiation of condylar bone marrow-derived mesenchymal stem cells (BMSCs). METHODS: Forty 4-week-old SD rats were randomly divided into the bilateral nasal obstruction group (28 rats) and the control group (12 rats). The animal experiment lasted for 4 weeks, from 8:00 to 16:00 every day. During modeling, bilateral condylar BMSCs were cultured in vitro, CCK-8 was used to compare the difference of cell proliferation,real-time polymerase chain reaction (RT-PCR) was used to detect the difference of gene expression of BMSCs chondrogenic marker genes (Agg, COL-Ⅱ, SOX -9). The data were statistically analyzed using SPSS19.0 software package. RESULTS: There was no significant difference of condylar BMSCs proliferation ability between the two groups (P>0.05), but the expression of chondrogenic marker genes were all significantly lower in the experimental group (P<0.05). CONCLUSIONS: It is suggested that the mechanism of mandibular deficiency caused by long-term mouth breathing is closely related to the inhibition of condylar BMSCs to cartilage differentiation.