美沙拉嗪、柳氮磺胺吡啶对葡聚糖硫酸钠诱导的Balb/c小鼠急性溃疡性结肠炎的治疗和免疫影响

目的：探讨美沙拉嗪和柳氮磺胺吡啶对葡聚糖硫酸钠（DSS）致Balb/c小鼠急性溃疡性结肠炎的作用效果和对急性溃疡性结肠炎免疫功能的影响。方法：Balb/c小鼠给予3.5% DSS水溶液自由饮用7 d建立溃疡性结肠炎模型，通过测定小鼠DAI指数、脏器指数、血清IL-4、结肠组织匀浆液IL-8的表达水平和小鼠体内CD4⁺细胞、CD8⁺细胞、CD4⁺CD25⁺细胞的变化，来评价两种药物对小鼠急性溃疡性结肠炎的作用。结果：给药治疗后，美沙拉嗪组和柳氮磺胺吡啶组的DAI评分和CMDI评分均降低（P<0.01或者P<0.05），小鼠症状缓解。结肠黏膜充血水肿减轻，且两种药物均能有效抑制病灶部位炎细胞的浸润。与模型组相比，美沙拉嗪组和SASP组CD3⁺细胞数增加，CD8⁺细胞数明显上升，CD4⁺CD25⁺细胞占CD4⁺细胞比例增加，血清中IL-4含量显著上升（P<0.01），组织中IL-8含量显著下降（P<0.01）；而美沙拉嗪组CD4⁺细胞数目略有增加，SASP组CD4⁺细胞数增加比较明显。结论：美沙拉嗪和柳氮磺胺吡啶可减轻DSS诱导的UC小鼠稀便、血便症状；两种药物对UC疾病的治疗可能与提高CD4⁺细胞水平和IL-4含量及上调CD8⁺细胞水平有关。

Therapeutic effects and immunization of mesalazine and SASP on active ulcerative colitis model of Balb/c mice induced by DSS

OBJECTIVE To investigate effects of mesalazine and SASP on active ulcerative colitis induced by DSS in Balb/c mice, and explore their effects on immunity of mice. METHODS Study animals were provided with distilled water containing 3.5% DSS for 7 days to induce UC. By measuring expression levels of the DAI, viscera index, serum IL-4, colonic tissues IL-8 and changes of CD4⁺, CD8⁺, CD4⁺CD25⁺ cells in mice, effects of mesalazine and SASP on active ulcerative colitis in mice were evaluated. RESULTS After administration of mesalazine and SASP medicine, DAI and CMDI scores of mice were reduced (P<0.01 or P<0.05), and signs and symptoms were improved. Hyperemia and edema of colonic mucosa were alleviated. Compared with UC mice, CD3⁺and CD8⁺ cellular levels were increased by mesalazine or SASP, CD4⁺CD25⁺ T percentage of CD4⁺ cells was also increased. CD4⁺ cells of SASP group were increased more obviously than in mesalazine group. Both mesalazine and SASP obviously increased serum IL-4 content and significantly reduced IL-8 levels. CONCLUSION Mesalazine and SASP can alleviate DSS induced UC symptoms, including diarrhea and mucosanguineous feces. Therapeutic effects of mesalazine and SASP may be related to increased levels of CD4⁺cells, CD8⁺ cells and IL-8.