大孔吸附树脂优选短筒兔耳草总黄酮的纯化工艺

目的:研究大孔吸附树脂分离纯化短筒兔耳草总黄酮的最佳工艺条件。方法:采用紫外分光光度法和高效液相色谱法分别测定总黄酮质量浓度和木犀草素含量,从而考察8种不同类型的大孔吸附树脂(AB-8,DM130,DM301,S-8,NKA-9,D101,HPD100,H103)的吸附和解吸附性能。结果:AB-8型大孔树脂对短筒兔耳草总黄酮有较好的吸附和洗脱效果,其最佳分离纯化条件:上样液吸附pH值为3.81,上样液质量浓度为0.58 mg·mL^-1,树脂对上样液的吸附量为66 mL·g^-1,上样体积流量为1 mL·min^-1,依次用500 mL水洗脱,50 mL 65%乙醇洗脱。经AB-8树脂处理后的总黄酮的质量分数达67.39%、收率为94.73%。结论:AB-8型大孔吸附树脂用于富集纯化短筒兔耳草总黄酮效果较佳,优化后工艺条件稳定可行,适用于短筒兔耳草总黄酮的分离纯化。

Optimization of purification technology for total flavonoids from Lagotis brevituba Maxim by macroporous resin

OBJECTIVE To investigate purification technology of total flavonoids of Lagotis brevituba Maxim by macroporous resin.METHODS Eight macroporous resins were chosen with static and dynamic adsorption and desorption experiments to optimize purification parameters by using ultraviolet spectrophotometry (UV) and high performance liquid chromatography (HPLC) to determine total flavonoids and luteolin.RESULTS AB-8 macroporous resin was found to have good adsorption and desorption effects. Optimal purification conditions:pH value 3.81, sample mass concentration 0.58 mg·mL^-1, loaded amount 66 mL·g^-1, and loading flow rate 1 mL·min^-1. Sample was eluted by 50 mL each of water and 65% ethanol, respectively. Purity of total flavonoids increased to 67.39% after purification, and yield was 94.73%.CONCLUSION AB-8 is an ideal resin with the best enrichment for separating and purifying total flavonoids of Lagotis brevituba Maxim. This optimized process is stable, feasible and suitable for separation and purification of total flavonoids from Lagotis brevituba Maxim.