CD16参与大鼠外周神经损伤及痛觉过敏的研究

目的:观察实验性大鼠神经损伤过程中CD16在三叉神经节的动态表达和分布,探讨三叉神经系统通过神经免疫反应参与外周神经损伤及痛觉过敏的潜在机制。方法:建立大鼠眶下神经慢性压榨损伤动物模型,应用免疫荧光标记观察CD16在三叉神经节的表达和分布,通过与CGRP的相关性分析,检测痛觉过敏过程中CD16的参与作用。结果:大鼠眶下神经压榨性损伤72 h后,三叉神经节初级感觉神经元CD16的表达显著性增强,5 d后达峰值,且神经元内CD16的表达与CGRP的表达具有显著相关性。结论:三叉神经系统初级感觉神经元细胞表达CD16,并参与外周神经损伤和痛觉过敏过程。

Study on CD16 Involved in Injury of Peripheral Nerve and Associated Hyperalgesia in Rats.

Objective: To investigate the dynamic expression of CD16 in the trigeminal ganglion (TG), and elucidate the potential role of neuro-immune interaction in the trigeminal nervous system that involved in the histopathological development of nerve injury and associated hyperalgesia. Methods: A rat animal mode of chronic constriction injury (CCI) was induced by placing loose chronic gut ligatures around the right infraorbital nerve (ION). The dynamic expression of CD16 in TG was demonstrated by immunofluorescence. Furthermore, the effect of CD16 on the associated hyperalgesia was detected through the correlation analysis with CGRP. Results: The expression of CD16 in the TG primary sensory neurons increased significantly at 72 hours post ION-CCI and reached the peak after 5 days. The expression of CD16 in TG neurons was significantly correlated with CGRP. Conclusion: The primary sensory neurons in the trigeminal nervous system were expressed CD16 and may involve in the histopathological development of peripheral nerve injury and associated hyperalgesia.