大鼠正畸牙移动中NF-κB通路相关差异基因的GO分析

目的:利用基因表达谱芯片对正畸加力组与正常大鼠牙齿牙周组织的基因表达谱进行对比分析,筛选差异基因并进行GO分析。方法:将6只SD大鼠随机分为加力组和对照组,加力组建立大鼠正畸牙齿移动模型。根据预实验结果,分别在0 h、12 h处死大鼠,并截取上颌第一磨牙及外周约1 mm的牙周组织,提取总RNA,进行全基因组表达谱芯片检测,对差异表达基因进行GO分析,并运用RT-qPCR对芯片结果进行验证。结果:芯片结果显示,共筛选到463条差异基因,其中实验组与对照组相比250个基因表达上调,213个基因表达下调,在差异基因中涉及到NF-κB信号通路的基因有8个,均表达上调。GO分析涉及到1189个不同的功能分类。结论:正畸加力12h后牙齿牙周组织中与炎症反应相关的基因和与中性粒细胞趋化相关的基因表达上调。

GO Analysis of Differentially Expressed Genes of NF- kappa B Pathway in Rat Orthodontic Tooth Movement.

Objective: To compare the mRNA expression profiles of periodontal tissues surrounding tooth between normal rats and rats with orthodontic force, to screen different genes and perform GO analysis by microarray technology. Methods: Six SD rats were randomly selected and divided into experimental group and control group. The experimental group established rat orthodontic tooth movement model. According to the results of pre-experiment, the rats were sacrificed at 0h and 12h, respectively, and the maxillary first molar peripheral 1 mm periodontal tissues were harvested and sampled, then total RNA was extracted for rat whole genome microarray detection. GO analysis of differentially expressed genes was performed and RT-qPCR was used to confirm the results on core samples. Results: Microarray results showed that, in contrast to control group, a total of 463 differentially expressed genes, including 250 up-regulated genes and 213 down-regulated genes existed in the experimental group. Among the differentially expressed genes, there were 8 up-regulated genes involved in NF- kappa B signaling pathway. And the GO analysis involved 1189 different functional categories. Conclusion: Inflammation and neutrophil chemotaxis related genes up-regulated in periodontal tissue during orthodontics tooth movement.