PRF促进小鼠全层皮肤损伤修复的实验研究

目的:探讨富血小板纤维蛋白(PRF)对小鼠皮肤损伤的修复作用。方法:采用8~10周的Balb/C小鼠21只,将其分为3组,分别为皮肤缺损空白对照组,上市产品对照组(AD甲壳素),冻干异种PRF颗粒组,在背部制备1个1.5 cm圆形全层皮肤损伤创面。通过大体观察,测定伤口愈合时间和愈合率,并在第21天,取皮肤组织做病理切片,观察组织愈合情况。结果:伤后21 d,各组创面逐渐缩小,冻干异种PRF颗粒组创面面积明显小于其余各组(P＜0.01),并且愈合时间较其余各组短。结论:PRF可以明显促进创面愈合速度,提高愈合质量,为临床皮肤损伤修复提供新的治疗方法。

Repair of Skin Defect in Mice with Platelet-rich Fibrin.

Objective: To assess the possible protective effect of platelet-rich fibrin (PRF) against mice impaired wound. Methods: Twenty-one Balb/C mice, who received full thickness skin wound (1.5 cm × 1.5 cm) on the back were obtained. The wound mice were then randomly divided into 3 groups, i.e. skin defect control group, public products in the control group (AD chitin), and freeze-dried PRF particle groups. The healing time and the healing rate of wound in mice were measured in different groups. Skin tissue pathological slices were taken on 21th day and healing condition was observed. Results: On days 21, wound area and volume in freeze-dried PRF particle treatment wounds were significantly decreased compared with those in other groups (P＜0.01).The healing time was shorter than that of other groups. Conclusion: These results confirmed that PRF had potential to accelerate the speed and improve the quality of open wound healing of skin. It provides a new strategy to promote skin wound repair after injury.