Satb2过表达对牙髓干细胞增殖和迁移能力的影响

目的:通过慢病毒介导Satb2(special AT-rich binding protein 2,Satb2)感染人牙髓干细胞(human Dental pulp stem cells,hDPSCs),观察Satb2过表达对人牙髓干细胞迁移和增殖能力的影响。方法:构建Satb2过表达慢病毒感染人牙髓干细胞,通过筛选得到稳定过表达Satb2细胞克隆。CCK8实验检测过表达Satb2对人牙髓干细胞的增殖能力的影响。划痕实验和Transwell细胞迁移实验观察细胞迁移能力的变化。免疫荧光染色观察细胞骨架微管的变化。结果:过表达Satb2的人牙髓干细胞相比对照组增殖能力增强(P＜0.05),微管更粗大,细胞的迁移能力增强。结论:过表达Satb2能使人牙髓干细胞的增殖和迁移能力增强。

Effect of Satb2 Overexpression on Proliferation and Migration of Human Dental Pulp Stem Cells.

Objective: To study the influence of special AT-rich binding protein 2 (Satb2) overexpressionon the proliferation and migration of human dental pulp stem cells (hDPSCs). Methods: Lentiviral-mediated gene was used to transfer Satb2 overexpression into hDPSCs, while control groups were normal hDPSCs and hDPSCs infected with empty vector. Cellular proliferation was determined by CCK8 assay. Immunofluorescence staining was performed to observe the structure of microtubules. Scratch-wound assay and migration assay were used to detect the migration of Satb2-overexpressing hDPSCs. Results: After 1, 3, 5, 7 days of cultivation, the optical density (OD) in the Satb2-overexpressing hDPSCs group was significantly higher than that in the control groups (P＜0.05). The microtubules in Satb2-overexpressing hDPSCs group were more slender than those in the control groups. Scratch-wound and migration assay showed that overexpression of Satb2 promoted the migration of hDPSCs. Conclusion: Overexpression of Satb2 promoted the proliferation and migration of hDPSCs.