| 高效液相色谱法测定人血清中盐酸阿夫唑嗪浓度 |
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| 引用本文： | 胡玉钦,姜楠,柴爱军,刘会臣,侯艳宁.高效液相色谱法测定人血清中盐酸阿夫唑嗪浓度[J].中国药房,2001,12(7):415-416. |
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| 作者姓名： | 胡玉钦 姜楠 柴爱军 刘会臣 侯艳宁 |
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| 作者单位： | 1. 白求恩国际和平医院临床药理室
2. 石家庄市人民医院 |
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| 摘 要： | 目的 :采用高效液相色谱法测定人血清中盐酸阿夫唑嗪的浓度。方法 :分析柱 :Shim -packCLC -ODS(4 6mm×150mm ,5μm )流动相 :甲醇 -乙腈 -磷酸盐缓冲液 -三乙胺 (10∶30∶60∶0 05) ;流速 :1 0ml/min ;荧光激发波长 :334nm ,发射波长 :378nm ;血清中样品用乙酸乙酯提取。结果 :血清中盐酸阿夫唑嗪浓度在0 40～51 20μg/L范围内线性关系良好 ,回归方程为 :A=0 3909C +0 0605(n=8 ,r=0 9996)。高、中、低3个浓度盐酸阿夫唑嗪的日内RSD分别为6 71 %、4 02 %和6 11 % ,平均回收率为100 87 % ;日间RSD分别为10 83 %、6 78 %和13 64 % ,平均回收率为97 93 %。最低检测血药浓度 (LOD )为0 20μg/L。结论 :本法灵敏、准确 ,分析时间短 ,可用于药代动力学研究
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| 关 键 词： | 盐酸阿夫唑嗪 血药浓度 高效液相色谱 |
| 文章编号： | 1001-0408(2001)07-0415-02 |
| 修稿时间： | 2000年9月13日 |
Determination of alfuzosin in Human Serum by HPLC |
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| HU Yuqin,JIANG Nan,LIU Huichen,HOU Yanning.Determination of alfuzosin in Human Serum by HPLC[J].China Pharmacy,2001,12(7):415-416. |
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| Authors: | HU Yuqin JIANG Nan LIU Huichen HOU Yanning |
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| Abstract: | OBJECTIVE:Alfuzosin concentration in human serum was determined by RP-HPLC METHODS:The mobile phase was methanol-acetonitrile-phosphate buffer-triethylamine(10∶30∶60∶0 05) After being extracted with ethyl ace_tate,alfuzosin was analyzed by reversed-phase HPLC (Shim-pack CLC-ODS,4 6mm×150mm,5μm)and fluorescence detection with excitation wavelength set at 334nm and emission at 378nm RESULTS:Over the concentration range of 0 4～51 2μg/L,the linear regression equation was A=0 3 909C+0 0 605(n=8,r=0 9 996) The average recovery of alfuzosin was 99 4% The intra-and inter-day RSDs were less than 15% CONCLUSION:The method is accurate and can be used for studying the pharmacokinetics of alfuzosin |
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| Keywords: | alfuzosin human serum HPLC |
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