DBA／2 小鼠微小残留白血病模型的构建

 目的　探讨建立一种细胞系和种鼠传代方便、重复性好的微小残留白血病模型的方法。方法　选用国际标准近交系DBA／2小鼠84只，接种不同数量L1210白血病细胞，观察接种白血病细胞数量与小鼠存活时间之间的关系；每只小鼠接种L1210白血病细胞1×106个后，第3天行不同剂量环磷酰胺（CTX）化疗，观察化疗剂量与小鼠存活时间之间的关系。结果　小鼠存活时间随接种细胞数量增加而逐渐缩短；白血病小鼠存活时间随化疗剂量增加而逐渐延长；观察接种不同数量白血病细胞的小鼠和接受不同剂量CTX化疗的白血病小鼠的生存趋势，显示接种500个白血病细胞小鼠的存活时间相当于用CTX剂量125 mg／kg化疗白血病小鼠生存时间，两者均为28 d左右。结论　DBA／2小鼠接种L1210白血病细胞1×106个／只后第3天采用CTX 125 mg／kg化疗可以成功建立微小残留白血病模型，此时小鼠体内白血病细胞数约为500个。

Model establishment of DBA/2 mouse minimal residual leukemia

Objective To establish a cell line and a convenient and well-duplicated minimal residual leukemia (MRL) model. Methods We chose the international standard domestic inbred DBA/2 mouse to inoculate the different numbers of L1210 leukemic cells to investigate the relationship between inoculating cell number and mouse survival time. On the third day after 1×106 leukemic cells per mouse were inoculated, different doses of CTX were used for the chemotherapy. Results The inoculating number of L1210 leukemic cells increased while the mouse survival time decreased;The survival time increased following the increase of the doses of the CTX;The survival time of the inoculated mouse treated with CTX at 125 mg/kg was equal to that of the mouse inoculated with 500 L1210 leukemic cells without treatment. Conclusion The observation results are that the dose of 125 mg/kg for creating the model of MRL mouse.