多指标综合加权评分法优选五味子与丹参混合醇提工艺研究

目的通过多指标综合加权评分法优选五味子与丹参混合醇提工艺,确定最佳提取工艺参数;通过DAD检测器优选最佳测定波长。方法单独和混合提取采用90%、70%、50%乙醇回流提取。优化工艺采用正交试验法,以乙醇体积分数、溶剂倍数、提取时间和提取次数为考察因素,以五味子醇甲、五味子酯甲、五味子甲素、五味子乙素、丹参酮ⅡA及丹酚酸B的量为考察指标,采用HPLC法,DAD检测器进行检测,综合加权评分法进行数据分析。结果混合提取五味子和丹参药材有效成分的提取率比单独提取更高;最佳工艺参数为90%乙醇回流提取3次,溶剂倍数为8倍量,每次1.5 h。结论五味子与丹参混合提取工艺简单、可行,有效成分提取率更高。不同物质同时测定时采用不同波长可有效消除干扰,准确性更高。

Optimization of ethanol extraction technique for Schisandrae Fructus and Salviae Miltiorrhizae Radix by multi index comprehensive weighted score evaluation

Objective To optimaize the ethanol extraction technique for Schisandrae Fructus and Salviae Miltiorrhizae Radix and confirm the optimum extraction process parameters by multi index comprehensive weighted score evaluation. Methods Alone and mixed extraction was carried out by using 90%, 70%, and 50% ethanol relux extraction. Orthogonal test method was uesd to optimize the process with ethanol concentration, solvent ratio, extraction time, and extraction times as detecting factors and schisandrin, schisantherrin A, deoxyschisandrin, schisandrin B, Tanshinone II_A, and salvianolic acid B as examining indexes, the detection was carried out by HPLC and DAD detector. The data were analyzed by multi index comprehensive weighted score evaluation. Results The effective composition contents of mixed extract from Schisandrae Fructus and Salviae Miltiorrhizae Radix were more than alone extracting. The best extraction conditions were solvent ratio of 1:8 with 90% ethanol reflux extraction for three times, and 1.5 h each time. Conclusion The mixed extracting is simple and appropriate, the extraction yield of active ingredients by mixed extracting is more than alone extracting. Different materials with different wavelength can effectively eliminate the interference at the same time, the accuracy is higher.