双苓扶正抗癌制剂对SGC-7901细胞增殖及c-myc基因表达的研究

目的：研究双苓扶正抗癌制剂对人胃癌细胞增殖及c-myc基因表达的影响。方法：采用MTT比色法,观察双苓扶正抗癌制剂（40～640 μg·mL^-1）5个剂量组单用及其与阿霉素（0.4,4.0 μg·mL^-1）及顺铂（0.1,1.0 μg·mL^-1）分别合用在体外对人胃癌SGC-7901细胞增殖的抑制作用；采用流式细胞技术检测该制剂（80～320 μg·mL^-1）对c-myc基因阳性蛋白标记率的影响。结果：双苓扶正抗癌制剂单用体外作用24 h,对人胃癌细胞增殖具有抑制作用,在40～640 μg·mL^-1剂量下,其抑制率随剂量增加而增加；与阿霉素(0.4,4.0 μg·mL^-1)或顺铂(0.1,1.0 μg·mL^-1)分别合用,可提高对人胃癌细胞的抑制率；在80～320 μg·mL^-1剂量下,可抑制c-myc基因的表达。结论：双苓扶正抗癌制剂单用对人胃癌细胞增殖具有抑制作用,且具有量效关系；与阿霉素或顺铂分别合用对人胃癌细胞的抑制作用具有协同效应；下调c-myc基因的表达可能是该制剂抑制肿瘤细胞增殖的作用机制之一。

Study of Shuangling Fuzheng anti-tumor preparation on proliferation and c-myc gene expression of SGC-7901 cells

OBJECTIVE: To study the effect of Shuangling Fuzheng anti-tumor preparation (SLAP) five groups on proliferation and c-myc gene expression of SGC-7901 cells in vitro. METHOD: The inhibitory effect of single SLAP (40 -640 microg x mL(-1)) and combined therapy with adriamycin (0.4, 4.0 microg x mL(-1) or cisplatin (0.1,1.0 microg x mL(-1) on human gastric carcinoma SGC-7901 cells proliferation were observed by MTT colorimetric analysis method. Technique of flow cytometry in vitro was used to measure the rate of positive sign of SLAP (80 - 320 microg x mL(-1)) on c-myc gene protein of SGC-7901 cells. RESULT: SGC-7901 cells proliferation were inhibited by single SLAP in dose of 40 - 640 microg x mL(-1) 24 h. Its inhibitory rate was increased with increase of dose. The inhibitory rate on SGC-7901 cells could be increased by SLAP in dose of 40 - 640 microg x mL(-1) plus adriamycin in dose of 0.4 and 4.0 microg x mL(-1) or plus cisplatin in dose of 0.1 and 1.0 microg x mL(-1). At the same time, SLAP (80 - 320 microg x mL(-1)) also could inhibite the expression of c-myc gene of SGC -7901 cells. CONCLUSION: Single SLAP had inhibiting effect on human gastric carcinoma cells proliferation with a dose-effect relationship and synergic effect while combined with adriamycin or cisplatin. To inhibit the expression of c-myc gene of human gastric carcinoma cells might be one of action mechanisms of SLAP, which inhibited tumor cells proliferation.