格列喹酮对糖基化终产物诱导的人肾系膜细胞RANTES表达的影响

目的探讨格列喹酮对糖基化终产物（AGEs）诱导的人肾系膜细胞（HRMC）正常T细胞表达分泌的调节活化蛋白（RANTES）表达的影响。方法运用糖基化修饰的牛血清白蛋白（AGE-BSA）和格列喹酮干预体外培养的HRMC,用半定量RT-PCR检测细胞中RANTES mRNA水平,用ELISA法测定细胞培养上清中RANTES蛋白水平。结果格列喹酮干预组HRMC RANTES的mRNA表达和蛋白分泌低于AGE-BSA组（P〈0.05）,且降低水平呈浓度和时间依赖性。结论格列喹酮能抑制糖基化终产物诱导的HRMC RANTES的表达和分泌。

Effects of gliquidone treatment on the AGEs -induced RANTES expression of human renal mesangial cells

Objective To investigate the effects of gliquidone on the AGEs （advanced glycation end products） induced RANTES（Regulated upon activation,normal T-cell expressed and secreted） expression of human renal mesangial cells （HRMC）. Methods AGEs were prepared by incubation of bovine serum albumin （BSA） with high concentration of glucose at 37℃ in vitro. HRMC was cultured in the presence of AGE-BSA （glucose at 50 mmol/L） with or without gliquidone. RANTES mRNA was analyzed by semiquantity RT-PCR. The concentration of RANTES in the supernatant was quantified by ELISA. Results Gliquidone significantly inhibited the expression and secretion of RANTES induced by AGE BSA. The inhibition of RANTES expression and secretion was in dose and time dependent manners. Conclusions AGEs is a potential toxin to induce expression of RANTES in HRMCs, which is inhibited by gliquidone.