Fas蛋白在鼠急性脊髓组织损伤中分布特点的初步研究

目的 :探讨大白鼠急性脊髓损伤后损伤区Fas蛋白的表达及其分布特点。方法 :Wistar雌性大鼠 4 2只 ,使用改良Allen氏法制作急性脊髓损伤模型 36只 ,采取HE、Nissl染色和免疫组化方法 ,检测损伤后 1 ,4 ,8,2 4 ,72h及 7d的脊髓组织。结果 :在损伤后 4h ,损伤段灰质可见Fas阳性细胞 ,8h表达达高峰。正常组未见Fas阳性细胞表达。高倍镜观察 ,阳性细胞具有凋亡细胞的特征。结论 :Fas蛋白可能在诱导脊髓损伤后神经细胞凋亡的过程中 ,发挥着重要作用

The Expression of Fas Protein after Acute Rat Spinal Cord Injury

Objective: To determine the expression of Fas in the rat injuried spinal cord. Methods: Tissue samples were selected from 36 female Wistar rats model of spinal cord injury with Allen's method at the level of T 8 and T 9,and rats were killed at 1,4,8,24,72 h and 7 d after injury (n=6 pergroup). Six additional normal rats were used to investigate the distibution of Fas in the normal spinal cord. Three segments of every spinal cord were cut for morphological studies, including hematoxylin and eosin staining, Nissl and immunohistochemical staining. Results: Fas immunoreactivity was present in grey matter of the injuried region after 4 h,and with a maximum presence at 8 h. Typical apoptotic morphology was detected by microscopy in the positive cells. Six additional normal rats did not show any Fas immunoreactivity. Conclusion: The Fas may play an important role in induction of neuron and glial cells to apoptosis.