| Fas/FasL在再生障碍性贫血患儿中的表达及意义 |
| |
| 引用本文： | 赵晓庆,张宝玺,吴晓莉. Fas/FasL在再生障碍性贫血患儿中的表达及意义[J]. 实用儿科临床杂志, 2007, 22(15): 1152-1153 |
| |
| 作者姓名： | 赵晓庆 张宝玺 吴晓莉 |
| |
| 作者单位： | 河北医科大学第二医院,儿科,石家庄,050000 |
| |
| 摘 要： | 目的研究凋亡基因Fas及其配体(FasL)在儿童再生障碍性贫血(AA)中的表达及意义,探讨AA的发病机制。方法30例AA患儿分为重型再生障碍性贫血(SAA)组12例,慢性型再生障碍性贫血(CAA)组18例,对照组为健康体检儿童10例。采用流式细胞术结合单克隆抗体荧光染色的方法测定AA患儿Fas/FasL表达水平及CD34 细胞数量。比较各组间表达差别。结果AA患儿Fas表达水平显著高于健康对照组(P<0.05),但SAA与CAA组Fas表达水平无差别(P>0.05);FasL表达水平在AA和健康对照组比较无差异(P>0.05)。CD34 细胞数量AA组明显低于健康对照组(P<0.05)。结论Fas/FasL系统参与诱导AACD34 细胞凋亡过程,导致造血干细胞减少,骨髓造血衰竭。
|
| 关 键 词： | 贫血,再生障碍性 CD34 细胞 流式细胞术 儿童 |
| 文章编号： | 1003-515X（2007）15-1152-002 |
| 修稿时间： | 2007-06-18 |
Expression of Fas/FasL in Children with Aplastic Anemia and Its Clinical Significance |
| |
| ZHAO Xiao-qing,ZHANG Bao-xi,WU Xiao-li. Expression of Fas/FasL in Children with Aplastic Anemia and Its Clinical Significance[J]. Journal of Applied Clinical Pediatrics, 2007, 22(15): 1152-1153 |
| |
| Authors: | ZHAO Xiao-qing ZHANG Bao-xi WU Xiao-li |
| |
| Affiliation: | Department of Pediatrics, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, China |
| |
| Abstract: | Objective To investigate the expression of Fas/FasL in children with aplastic anemia(AA) and its clinical significance.To explore the pathogenesis of AA.Methods CD34 cell counts and the expression of Fas/FasL in 12 children with severe AA(SAA),18 with chronic AA(CAA)and 10 normal children were detected by flow cytometry combining monocolon antibody.Results The expression of Fas in children with AA were significantly higher than that in control group(P<0.05);The CD34 cell counts in AA were lower than that in control group(P<0.05);however the expression of FasL in children with AA and control group showed no significant difference(P>0.05).Conclusions Fas/FasL systeam take part in inducing CD34 cell apoptosis.This may contribute to understanding the decrease number of stem cells and bone marrow failure. |
| |
| Keywords: | Fas FasL |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
