| 高效液相色谱法测定补益活络丸中2,3,5,4,-四羟基二苯乙烯-2-O-β-D葡萄糖苷的含量 |
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| 引用本文： | 周琳. 高效液相色谱法测定补益活络丸中2,3,5,4,-四羟基二苯乙烯-2-O-β-D葡萄糖苷的含量[J]. 实用医药杂志(山东), 2008, 25(10) |
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| 作者姓名： | 周琳 |
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| 摘 要： | 目的建立高效液相色谱法测定补益活络丸中2,3,5,4,-四羟基二苯乙烯-2-O-β-D葡萄糖苷的含量。方法采用Hypersil ODSC18(4.6mm×200mm,5μm)色谱柱,乙腈-水(18∶82)为流动相,流速为1.0ml/min,检测波长320nm。结果2,3,5,4,-四羟基二苯乙烯-2-O-β-D葡萄糖苷在0.164～1.312μg范围内呈良好的线性关系,r=0.9997,平均回收率为99.80%(n=6),RSD=1.11%。结论所建立的方法稳定、可靠,可为该产品建立质量控制方法提供参考。
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| 关 键 词： | 高效液相色谱法 补益活络丸 2,3,5,4,-四羟基二苯乙烯-2-O-β-D葡萄糖苷 |
HPLC determination of 2,3,5,4,-Terahydroxy distyrene-2-O-β-D glucoside in Buyihuoluo Pills |
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| ZHOU Lin. HPLC determination of 2,3,5,4,-Terahydroxy distyrene-2-O-β-D glucoside in Buyihuoluo Pills[J]. Practical Journal of Medicine & Pharmacy, 2008, 25(10) |
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| Authors: | ZHOU Lin |
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| Abstract: | Objective To establish a HPLC method for determination of 2,3,5,4,-terahydroxystilbene -2-O-β-D glucoside in Buyihuoluo Pills. Methods A Hypersil ODSC18 column(4.6mm×200mm,5μm)was used with a mobile phase of acetonitrile-water(18∶82), the flow rate was 1.0ml/min, detection wavelength at 320nm. Results The linearity range was 0.164～1.312μg, r=0.9997, average recovery rate was 99.80%(n=6), RSD=1.11%. Conclusion This method is accurate and reproducible, and availed for quality control the Buyihuoluo Pills. |
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| Keywords: | 2 3 5 4 |
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