17β—雌二醇对白细胞介素6诱导的内皮细胞损伤的影响

目的 研究17β－雌二醇对白细胞介素6（IL－6）诱导的血管内皮细胞（VEC）损伤的保护作用。方法 以体外培养的人静脉内皮细胞（HUVEC）为模型。将IL－6与HUVEC孵育及HUVEC经17β－雌二醇处理或与它莫西芬（Tamoxifen)处理后加IL－6孵育，检测培养液中乳酸脱氢酶（LDH）、血管紧张素转换酶（ACE）含量，以反映细胞损伤程度。结果 IL－6组培养液中ACE、LDH浓度明显增加，且呈剂量依赖关系；经17β-雌二醇预处理后，细胞在IL－6作用下，ACE、LDH浓度无明显变化；经它莫西芬、17β－雌二醇预处理后再加IL－6组，功能变化与损伤组相似。结论 IL－6对血管内皮细胞的激活、损伤作用，此作用有剂量依赖关系。雌激素可保护IL－6引起的VEC损伤，其保护作用可它莫西芬拮抗。

The effect of 17

Objective The protection effects of 17β-e s tradiol on vascularendothelial cells(VECs) injured by interleukin-6 were stud ied.Methods Lactate dehydrogenase(LDH) release and angiotensi n converting enzyme(ACE) level were measured.Results Interleukin-6(IL-6) can increase production of ACE and LDH.When 17β-estradiol was added to culture media before IL-6，the le v el of ACE and LDH showed normal.However,when tamoxifen and 17β-estradiol were added,level of ACE and LDH were similar with the group of IL-6.Conclusions IL-6 can induce the endothelial cells injury in dose dependent manner.Estradiol is able to protect VECs injury by IL-6.B ut tamoxifen is able to block the effects.