低频限制性位点聚合酶链反应研究耐甲氧西林金黄色葡萄球菌的暴发流行

目的：探讨院内感染耐甲氧西林金黄色葡萄球菌（MRSA）流行状况,了解流行株耐药状况。方法：用K-B纸片法检测21株院内感染金黄色葡萄球菌耐药状况,建立本实验室低频限制性位点聚合酶链反应（IRS-PCR）分型方法。应用IRS-PCR对21株金黄色葡萄球菌进行基因分型。结果：21株菌经药敏实验分出6个耐药表型a～f,其中a型12株,均为多重耐药的MRSA。21株菌经IRS-PCR分为6个基因型A～F,其中A型12株来自烧伤病房,B、C、D、E、F型来源于重症监护室。结论：MRSA多为多重耐药,交叉感染是MRSA流行的主要原因。IRS-PCR能对金黄色葡萄球菌简易快速可靠分型。

Study of outbreak of methicillin-resistant staphylococcus aureus by using infrequent-restriction-site polymerase chain reaction

Objective:To explore the reason of outbreak of methicillin-resistant staphylococcus aureus(MRSA)and estimate the drug-resistant situation of epidemic strains.Methods:Drug-resistant situation of 21 hospital aquired strains was examined by K-B disk test.Twenty one strains were determined by infrequent restriction site polymerase chain reaction(IRS-PCR).Results:Twenty one staphylococcus aureus were sorted into 6 drug-resistant phenotypes by susceptibility test a-f.Type a 12 strains were all multidrug -resistant MRSA. Twenty one HA strains were sorted in to 6 types by IRS-PCR:A-F.Type A were collected from burn ward,type B,C,D,E,F were collected from ICU.Conclusion:MRSA is always multidrug -resistant.IRS-PCR is an easy,quick and dependable typing method for staphylococcus aureus. Cross-infection is the main reason of hospital aquired infection.