Isolation, amino acid sequence and action of guinea-pig ACTH on aldosterone production by glomerulosa cells |
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| Authors: | A I Smith C A Wallace R L Moritz R J Simpson L B Schmauk-White E A Woodcock J W Funder |
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| Affiliation: | Medical Research Centre, Prince Henry's Hospital, Melbourne, Australia. |
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| Abstract: | Though minor sequence differences between-species have been reported for adrenocorticotrophin, ACTH(1-39), the steroidogenic moiety ACTH(1-24) has appeared invariant in mammals. We here report the isolation, purification and amino acid sequencing of guinea-pig (GP) ACTH in which Pro24 is replaced by Ala24, and the demonstration that GP-ACTH stimulates aldosterone production to maximal levels well above those seen with human ACTH(1-39) or Synacthen, ACTH(1-24) amide, the synthetic ACTH fragment widely used for diagnostic and therapeutic purposes. |
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