高压氧对短暂前脑缺血小鼠海马脑红蛋白表达的影响

目的 观察小鼠脑缺血再灌注以及高压氧治疗后海马脑红蛋白表达的变化,探讨高压氧对脑红蛋白表达的影响及其意义.方法 雌性C57B/6N小鼠随机分为假手术对照组、缺血再灌注48h组(48h I/R组)及缺血再灌注48h加高压氧治疗组(48h HBO组).对各组小鼠脑组织神经元进行HE及免疫组织化学染色、图像学分析及统计学处理.结果 48h I/R组及48h HBO组的脑红蛋白表达均高于假手术组(P＜0.01和P＜0.001),48hHBO组吸光度高于48h I/R组(P＜0.01).结论 高压氧对缺血性脑损伤有显著治疗作用,提高脑红蛋白的表达水平可能是其治疗的机制之一.

EFFECT OF HYPERBARIC OXYGEN ON THE EXPRESSION OF NEUROGLOBIN IN MOUSE HIPPOCAMPUS AFTER TRANSIENT FOREBRAIN ISCHEMIA

Objective To study the effect and significance of hyperbaric oxygen treatment on the expression of neuroglobin by observing the changing tendency of neuroglobin in mouse hippocampus after forebrain ischemia-reperfusion. Methods Eighteen female C57BL/6N mice were divided into three groups randomly: the sham-operated control group,the 48-hour ischemia-reperfusion group(48h I/R) and the group treated with hyperbaric oxygen after 48-hour ischemia-reperfusion(48h HBO).HE staining and immunohistochemistry experiments were carried out on the brains of all the animals.Image analysis and statistical treatment were also performed for all the images. Results The neuroglobin expression level of 48h I/R group and the 48h HBO group were higher than that of the sham-operated group(P<0.01 and P<0.001,respectively);the optical density of the 48h HBO group was higher than that of the 48h I/R group(P<0.01).Conclusion Hyperbaric oxygen treatment had an obvious effect on ischemic brain injury,and increasing the expression of Neuroglobin might be one of the possible mechanisms of the therapy.