Comparison of molecular and conventional methods for typing of enteroviral isolates |
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| Authors: | Manayani Darly Joseph Shaji R V Fletcher G John Cherian Thomas Murali N Sathish N Solomon Tilak Gnanamuthu Chandran Sridharan Gopalan |
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| Affiliation: | Department of Clinical Virology, Christian Medical College and Hospital, Vellore-632004, India. |
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| Abstract: | Twenty-eight enteroviral isolates obtained from various clinical specimens were typed by Lim-Benyesh-Melnick (LBM) pool-based neutralization, PCR-restriction fragment length polymorphism (RFLP), and partial sequencing of the VP1 region of the enteroviral genome. Sequencing was found to be a good alternative to LBM typing, while PCR-RFLP was inadequate for identification of enteroviral isolates. |
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