胃癌组织中基质金属蛋白酶-2和E-钙黏附素检测

目的:探讨胃癌组织中基质金属蛋白酶 -2(MMP- 2)和E 钙黏附素 (E- CD)的表达。方法:采用原位杂交法,检测 72例胃癌(其中复发 39例)组织中MMP- 2mRNA和E- CDmRNA的表达,并观察 2者与浸润深度和淋巴结转移之间的关系。结果:胃癌组织中MMP -2mRNA和E CDmRNA的阳性表达与浸润深度和淋巴结转移有关。复发患者的 2种胃癌组织 (复发前手术切除标本;本次胃镜活检标本)与非复发胃癌组织标本的MMP 2mRNA和E- CDmRNA的阳性表达率差异有统计学意义(MMP 2:P<0. 01;E CD:P<0. 05)。MMP- 2mRNA阳性表达的胃癌组织中E CDmRNA的阳性表达率低于MMP 2mRNA阴性表达的胃癌组织, 2者呈负相关 (r=-0. 273,P<0. 05)。结论:MMP- 2mRNA和E- CDmRNA的表达与胃癌的浸润、转移及复发有关。检测MMP- 2mRNA和E- CDmRNA在胃癌患者胃镜活检组织中的表达可能有助于判断预后及胃癌转移复发潜能。

Expression of metalloproteinase-2 mRNA and E-cadherin mRNA in gastric carcinoma tissue

Aim: To evaluate the expression of metalloproteinase-2 (MMP-2)mRNA and E-cadherin (E-CD ) mRNA in gastric carcinoma tissues. Methods: The MMP-2 mRNA and E-CD mRNA were detected by hybridization in situ in 72 cases of gastric carcinoma, among which, 39 were recurrent cases. Results: The MMP-2 mRNA and E-CD mRNA levels were associated with invasive depth and lymph node metastasis. Compared with nonrecurrent group (30.4%),the MMP-2 mRNA expression was much higher in recurrent gastric carcinoma, which included recurrent specimen group (84.6%) and recurrent bioptic specimen group (92.3%)(P<0.05). The E-CD mRNA expression in bioptic specimen group(12.8%) was lower than that in non-recurrent group(33.3%)(P<0.05). The E-CD mRNA expression in MMP-2 mRNA positive gastric carcinoma tissue was much lower than that in negative carcinoma tissue.Conclusion: Expression of MMP-2 mRNA and E-CD mRNA is associated with invasion, metastasis and recurrence of gastric carcinoma. Detection of MMP-2 mRNA and E-CD mRNA in bioptic specimens may be helpful in predicting tumor metastatic and recurrent potential and prognosis for patients with gastric carcinoma.