| 甘肃产秦艽的DNA指纹图谱鉴别 |
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| 引用本文： | 徐红,王峥涛,胡之璧.甘肃产秦艽的DNA指纹图谱鉴别[J].国外医药(植物药分册),2008,23(3). |
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| 作者姓名： | 徐红 王峥涛 胡之璧 |
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| 作者单位： | 上海中医药大学中药研究所,中药标准化教育部重点实验室,上海,201203;上海中药标准化研究中心,上海,201203 |
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| 基金项目： | 上海市科委资助项目，上海市重点学科建设项目 |
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| 摘 要： | 目的 对产于我国甘肃省的中药秦艽的3种基源植物秦艽、麻花秦艽与小秦艽进行RAPD分析,建立具有鉴别意义的DNA指纹图谱.方法 采用RAPD分子标记对秦艽的基因组DNA进行PCR扩增,利用NTSYSpc 2.1和Popgene 3.2软件分析3种秦艽间的亲缘关系,构建树系图.结果 从80条RAPD引物中筛选出4条具有鉴别意义的多态性引物,在3种秦艽中共得到39个DNA条带,其中共有条带2条,多态条带37条.据此获得了能有效区别3种秦艽的多态性RAPD指纹谱.结论 甘肃产秦艽的3种基源植物遗传分化明显,RAPD方法能有效地将它们区分鉴别.
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| 关 键 词： | 秦艽 鉴别 遗传关系 |
Study on authentication of Radix Gentianae Macrophyllae from Gansu Province by DNA fingerprints |
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| Authors: | XU Hong WANG Zheng-tao HU Zhi-bi |
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| Institution: | 1;2;1;2;1;2;1.Key Laboratory of Standardization of Chinese Medicines of Education;Institute of Chinese Materia Medica;Shanghai University of Traditional Chinese Medicine;Shanghai 201203;China;2.Shanghai R & D Centre for Standardization of Chinese Medicines;China |
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| Abstract: | Objective To establish the DNA fingerprints for identification the three origin plants- Gentiana macrophylla,G.strarninea,and G.dahurica of Chinese traditional medicine"Qinjiao"(Radix Gentianae Macrophylla)from Gansu Province.Methods PCR was performed with the genomic DNA of Radix Gentianae Macrophyllae by RAPD marker,the genetic relationship among the Radix Gentianae Macrophylla was analyzed and the DNA molecular dendrogram was set up by the software NTSYSpc 2.1 and Popgene 3.2.Results The four effective p... |
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| Keywords: | RAPD |
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