| 三氧化二砷对人肺癌A549细胞凋亡及耐药基因表达的影响 |
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| 引用本文： | 郑东庆,薄挽澜,梁桃. 三氧化二砷对人肺癌A549细胞凋亡及耐药基因表达的影响[J]. 中国基层医药, 2010, 17(1): 7-9,I0003. DOI: 10.3760/cma.j.issn.1008-6706.2010.01.003 |
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| 作者姓名： | 郑东庆 薄挽澜 梁桃 |
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| 作者单位： | 1. 大庆油田总医院呼吸科,黑龙江省大庆,163001
2. 哈尔滨医科大学第一临床医学院消化科 |
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| 摘 要： | 目的研究三氧化二砷（As2O3）对人肺癌细胞株的诱导凋亡作用及对耐药基因LRP表达的影响。方法应用人肺腺癌A549细胞株，运用体外细胞培养法、MTT法、流式细胞术检测As2O3对人肺癌细胞株抑制及诱导凋亡作用；用逆转录-聚合酶链反应（RT-PCR）方法检测LRP mRNA的表达。结果As2O3对人肺癌A549细胞具有抑制作用，其抑制率呈时间-剂量依赖关系。不同浓度的As2O3均可诱导凋亡。1．0μmol／L、3．0μmol／L的As2O3可下调LRPmRNA的表达。结论As2O3具有抑制肿瘤细胞生长及诱导细胞凋亡作用，其机制与下调LRPmRNA表达有密切关系。
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| 关 键 词： | 三氧化二砷 肺肿瘤 基因 细胞凋亡 |
Effect and mechanism of arsenic trioxide on LRP mRNA expression in human lung adenocarcinoma cells in vitro |
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| ZHENG Dong-qing,BO Wan-lan,LIANG Tao. Effect and mechanism of arsenic trioxide on LRP mRNA expression in human lung adenocarcinoma cells in vitro[J]. Chinese Journal of Primary Medicine and Pharmacy, 2010, 17(1): 7-9,I0003. DOI: 10.3760/cma.j.issn.1008-6706.2010.01.003 |
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| Authors: | ZHENG Dong-qing BO Wan-lan LIANG Tao |
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| Affiliation: | . ( Department of Respiration, Youtian Hospital of Daqing, Daqing, Heilongjiang 163001, China ) |
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| Abstract: | Objective To study the mechanism of arsenic on human lung adenocarcinoma cells.Methods MTT,FCM and RT-PCR tests were used to investigate apoptosis levels induced by arsenic trioxide and the effect on LRP mRNA expression.Results 1.0μmol/L and 3.0μmol/L arsenic trioxide can decrease LRP mRNA expression.Conclusion Low concentration As_2O_3 may improve apoptosis of lung caner by decreasing LRP mRNA expression.As_2O_3 may be used to treat lung cancer but the results are not better than cisplatin. |
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| Keywords: | Arsenic trioxide Lung neoptasms Genes Apoptosis |
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