DREAM A和B在原代培养的小鼠神经细胞中的表达

目的:研究DREAM蛋白的2个主要亚型DREAM A和DREAM B在原代培养的小鼠大脑皮层星形胶质细胞、小鼠大脑皮层γ-氨基丁酸能神经元以及小鼠小脑谷氨酸能神经元中的表达水平的差异。方法:提取原代培养的小鼠大脑皮层星形胶质细胞、小鼠大脑皮层γ-氨基丁酸能神经元以及小鼠小脑谷氨酸能神经元的RNA,采用普通PCR及real time PCR的方法检测上述各种细胞中DREAM蛋白2个亚型A和B的mRNA的表达水平。结果:DREAM的两个亚型A和B在上述三种神经细胞都存在。在发育成熟的原代培养细胞中,DREAMA的mRNA水平要远高于DREAM B。在三种不同的细胞中,谷氨酸能神经元表达DREAM A的水平最高。结论:DREAM的两个亚型A和B在小鼠三种原代培养的神经细胞中的表达水平及其差异提示DREAM A是成年小鼠中DREAM的主要亚型,DREAM A和B在不同类型的神经细胞中发挥的作用及其机制有所不同。

Expression of DREAM A and DREAM B in primary cultured nerve cells of mice

Objective: To study the difference of the expression level between the two main isoforms of DREAM A and DREAM B in the primary cultured cerebral cortical astrocytes,GABAergic nerons and cerebellar glutamatergic neurons of the mice.Methods: RNA of the above three primary cultured cells from mice were extracted and then detected about the DREAM A and DREAM B 's mRNA level by the polymerase chain reaction(PCR) and real time PCR.Results: Both DREAM A and DREAM B existed in the above three nerve cells.In the mature primary culture cells,the mRNA level of the DREAM A was higher than that of DREAM B,and the expression level of DREAM A of the glutamatergic neurons was the highest among the three cells.Conclusion: The expression levels and differences of the DREAM A and DREAM B show that DREAM A was the main type of DREAM in adult mice,DREAM A and DREAM B may play different roles throug diffenent action mechanisms in different types of nerve cells.