重复性经颅磁刺激与舍曲林改善CUMS大鼠焦虑抑郁样行为的比较及其机制研究

目的:比较重复性经颅磁刺激(repetitive transcranial magnetic stimulation,rTMS)和舍曲林处理对慢性不可预见温和应激(chronic unpredictable mild stress,CUMS)大鼠焦虑抑郁样行为的改善作用及其可能的作用机制。方法:32只SD大鼠随机分为对照组、CUMS组、CUMS+rTMS组以及CUMS+舍曲林组,每组8只。采用糖水偏好实验、强迫游泳实验及旷场实验观察各组大鼠行为,并以蛋白质印迹法(Western blot)检测各组大鼠海马内源性大麻素I型受体(cannabinoid type 1 receptor,CB1R)的表达。结果:CUMS组糖水偏好值、水平活动度、中央活动次数以及CB1R的表达水平均显著低于对照组、CUMS+rTMS组和CUMS+舍曲林组,而CUMS+rTMS组和CUMS+舍曲林组两者之间没有统计学差异;CUMS组的强迫游泳不动时间大于其它三组,而CUMS+rTMS组和CUMS+舍曲林组两者之间没有统计学差异。结论:rTMS与舍曲林均能改善CUMS大鼠的焦虑抑郁样行为,二者的改善作用没有统计学差异,其作用可能是通过上调海马CB1R的表达实现的。

The comparison of rTMS and sertraline ameliorate anxiety-and depression-like behaviors in a rat model of CUMS and the possible mechanism

Objective: To compare the effects and the underlying mechanisms of repetitive transcranial magnetic stimulation(rTMS) and sertratline administration in ameliorating anxiety-and depression-like behaviors in a rat model of chronic unpredictable mild stress(CUMS).Methods: 32 adult male SD rats were randomly divided into control,CUMS,CUMS+rTMS and CUMS+sertraline groups(n=8 for each group).The behavior of rats were investigated by sucrose preference test,forced swimming test and open-field test,and the expression of cannabinoid receptor type 1 receptor(CB1R) in hippocampus was measured by Western blot.Results: The sucrose preference percentage,horizontal motion distance,the number of entering central region and the expression of CB1R in CUMS group was less than those in control,CUMS+rTMS and CUMS+sertraline groups,but there was no significant difference between CUMS+rTMS group and CUMS+sertraline group;the immobility time in CUMS group was longer than that in other three groups,but there was no significant difference between CUMS+rTMS group and CUMS+sertraline group.Conclusion: Both rTMS and sertraline can improve anxiety and depression behaviors of CUMS rats with no significant difference between the two,the effects of rTMS and sertralin may be mediated by up-regulation of the expression of CB1R.