| CD3AK杀伤机理及其杀伤活性调控效应的初步研究 |
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| 引用本文： | 刘永忠,张玲,王芸,郭鸣,毛海婷.CD3AK杀伤机理及其杀伤活性调控效应的初步研究[J].中国肿瘤生物治疗杂志,1997,4(4):294-297. |
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| 作者姓名： | 刘永忠 张玲 王芸 郭鸣 毛海婷 |
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| 作者单位： | 山东省医学科学院基础所免疫室 济南250062 |
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| 摘 要： | 应用抗CD3单抗和人rIL-2共同诱导人外周血单个核细胞制备人CD3AK细胞(CDAK).采用LDH释放法、ABC-CELISA法、流式细胞术等方法观察了CD3AK杀伤癌细胞的机理及人rIFN-α、rIFN-γ、rTNF细胞因子、化疗药顺氨氯铂(CDDP)和阿霉素(ADM)对CD3AK杀伤活性的调控效应.结果表明,①粘附分子ICAM-1/LFA-1参与CD3AK的杀瘤过程,并且rIFN-α、rTNF的促CD3AK杀伤效应亦是通过上述途径实现的.③CD3AK通过分泌可溶性杀伤因子间接杀伤癌细胞.③CD3AK通过膜相关TNF参与杀伤效应.④CD3AK介导癌细胞凋亡.⑤化疗药CDDP和ADM处理癌细胞后,提高其对CD3AK杀伤活性的敏感性,CDDP促杀伤效应与其上调癌细胞表面ICAM-1、HLA-ABC抗原表达有关.
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| 关 键 词： | CD3AK 杀伤活性 粘附分子 HLA抗原 凋亡 |
| 收稿时间： | 1997/3/17 0:00:00 |
| 修稿时间： | 1997/6/27 0:00:00 |
Study on Mechanism and Regulation of CD3AK Cytotoxic Activity |
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| Liu Yongzhong,Zhang Ling,Wang Yun,Guo Ming and Mao Haiting.Study on Mechanism and Regulation of CD3AK Cytotoxic Activity[J].Chinese Journal of Cancer Biotherapy,1997,4(4):294-297. |
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| Authors: | Liu Yongzhong Zhang Ling Wang Yun Guo Ming and Mao Haiting |
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| Institution: | Department of Immunology, Institute of Basic Medical Sciences , Shandong Academy of Medical Sciences , Jinan 250062;Department of Immunology, Institute of Basic Medical Sciences , Shandong Academy of Medical Sciences , Jinan 250062;Department of Immunology, Institute of Basic Medical Sciences , Shandong Academy of Medical Sciences , Jinan 250062;Department of Immunology, Institute of Basic Medical Sciences , Shandong Academy of Medical Sciences , Jinan 250062;Department of Immunology, Institute of Basic Medical Sciences , Shandong Academy of Medical Sciences , Jinan 250062 |
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| Abstract: | |
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| Keywords: | CD3AK cytotoxicity adhesion molecule HLA antigen apoptosis |
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