| 中药提取物9901的体外抗肿瘤活性研究 |
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| 引用本文： | 刘艳,杜智敏,姜宝红,高焕焕,艾敬. 中药提取物9901的体外抗肿瘤活性研究[J]. 哈尔滨医科大学学报, 2001, 35(1): 37-38 |
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| 作者姓名： | 刘艳 杜智敏 姜宝红 高焕焕 艾敬 |
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| 作者单位： | 1. 哈尔滨医科大学附属第二医院 临床药学药物研究所
2. 哈尔滨医科大学 药理学教研室，黑龙江 哈尔滨 150086 |
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| 摘 要： | 目的 用体外培养法研究中药提取物9901对肿瘤HeLa细胞生长的抑制作用和作用机制。方法 采用MTT法和激光扫描共聚焦显微镜进行研究。结果 HeLa细胞在9901处理3天后，测得该药的IC50为5.12μg/ml。用不同浓度的9901处理HeLa细胞48h后，用PI染色，HeLa细胞的生长，通过影响细胞的DNA而发挥作用。
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| 关 键 词： | 抗肿瘤药 中药提取物 9901 HeLA细胞 体外培养 |
| 文章编号： | 1000-1905(2001)01-0037-02 |
| 修稿时间： | 2000-03-01 |
Anticancer effect of TCD-extract-9901 in vitro |
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| LIU Yan,DU Zhi min,JIANG Bao hong,et al. Anticancer effect of TCD-extract-9901 in vitro[J]. Journal of Harbin Medical University, 2001, 35(1): 37-38 |
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| Authors: | LIU Yan DU Zhi min JIANG Bao hong et al |
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| Abstract: | Objective To study the effect of TCD(traditional Chinesedrug)-extract-9901 on growth inhibition in HeLa cancer cell and the action mechanism of drug.Methods The parameters were got by MTT assay and laser scanning confocal microscope.Results The IC50 of this extract was 5.12μg/ml after HeLa cell was treated with 9901 for 3 days.The HeLa cell nucleus with PI staining varied and the DNA contents decreased significantly after HeLa cell were treated with different concentration 9901 for 48 hours.With the statistics test,the DNA contents in treated and control goups were obviously different.Conclusion TCD-extract-9901 can effectively inhibit growth of HeLa cancer cell.The action mechanism of the herb's extract 9901 on HeLa cancer cell may be related with the DNA content in HeLa cell. |
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| Keywords: | antineoplastic agents traditional Chinese drug extract 9901 HeLa cell in vitro |
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