急性白血病及多发性骨髓瘤EB病毒DNA检测

目的用荧光定量PCR方法检测急性白血病（AL）与多发性骨髓瘤患者（MM）骨髓中EBV—DNA含量,并探讨其临床意义。方法采用荧光定量PCR技术检测40例急性白血病与25例多发性骨髓瘤患者EBV感染情况,同时检测了32例对照组小细胞低色素性贫血和巨幼细胞性贫血患者骨髓EBV—DNA含量。结果多发性骨髓瘤患者EBV感染率为56%,急性白血病组EBV感染率为7.5%,对照组EBV感染率6.2%。AL组与对照组EBV—DNA阳性率无显著性差异（P〉0.05）,MM组与对照EBV—DNA阳性率有显著性差异（P〈0.01）,MM组与AL组EBV—DNA阳性率有显著性差异（P〈0.01）。结论 AL患者的发病与EB病毒感染关系不大,MM患者的发病与EB病毒感染有关;应用荧光定量PCR方法对MM患者骨髓中EBV—DNA含量进行检测,对于揭示MM的发病与EBV感染之间的相关性提供了敏感可靠的检测方法。

Detection for EB Virus DNA of Acute Leukemia and Multiple Myeloma

Objective To detect the bone marrow EBV-DNA content with Fluorescence quantitative PCR,in patients with acute leukemia（AL） and multiple myeloma（MM）,and investigate its clinical significance.Methods To measure bone marrow tumour EBV-DNG with fluorescence quantitative PCR in the patient of 40 acute leukemia and 25 acute multiple myeloma（MM）,and measure the content in 32 Comparison cases of small cells hypochromic anemia and megaloblastic anemia.Results EBV infection rate in multiple myeloma is 56%.7.5% in acute leukemia and 6.2% in the control group.The positive rate of EBV-DNA was no significant difference between AL group and the control group（P0.05）.The positive rate of EBV-DNA has significant difference between MM group and the control group（P0.01）.The positive rate of EBV-DNA was significant difference between AL group and MM group（P0.01）.Conclusion The incidence of AL patients have little to do with EB virus infection then,then MM patients have incidence with EB virus infection;Detecting the bone marrow EBV-DNA content with fluorescence quantitative PCR in patients with multiple myeloma（MM） is a sensitive and reliable detection method for revealing the correlation between the pathogenesis of MM and EBV infection.