陡脉冲电场对荷瘤Wistar大鼠免疫功能的影响

研究陡脉冲电场(Steep pulsed electric fields,SPEFs)对接种Walker256癌肉瘤的Wistar大鼠免疫功能的影响,探讨SPEFs治疗恶性肿瘤的机理。将30只大鼠随机分为三组:荷瘤对照组(接种肉瘤,不治疗)、处理组(接种肉瘤,SPEFs治疗)和正常对照组(接种等量生理盐水,不治疗),每组各10只。治疗前及治疗后每隔3 d用游标卡尺测量肿瘤最大直径。治疗后2周,用MTT法检测大鼠脾淋巴细胞转化率和NK细胞活性、ELISA法检测大鼠腹腔巨噬细胞分泌TNF-α的表达。实验结果表明,SPEFs能明显抑制恶性肿瘤的生长(P<0．01)、明显增强大鼠脾淋巴细胞的增殖反应(P<0．05)、显著提高大鼠NK细胞的活性(P<0．05)和大鼠腹腔巨噬细胞产生TNF-α的能力(P<0．05)。本实验显示,SPEFs在通过不可逆性电击穿杀伤肿瘤细胞的同时,也能诱发机体抗癌免疫反应,增强机体的免疫功能,为临床应用提供治疗机理。

Effect of Steep Pulsed Electric Fields on the Immune Response of Tumor-bearing Wistar Mice

This study sought to evaluate the effect of steep pulsed electric fields (SPEFs) on the immune response of Wistar mice inoculated with Walker256 sarcoma. Thirty mice were randomly divided into three groups: control group (group A, inoculated with Walker256 sarcoma, not treated), treatment group (group B, inoculated with Walker256 sarcoma, treated by SPEFs), and normal control group (group C, inoculated with normal saline, not treated). Tumor size was measured before and every 3 days after treatment by vernier caliper. MTT methods were used to assess the lymphocytes proliferation and the natural killer (NK) cells activity. TNF-a activity was measured by ELISA. Statistical analysis was performed utilizing the SPSS10.0 software package. The experiment results revealed that tumor growth was significantly inhibited in group B as compared with group A (P < 0.01), and that lymphocytes proliferation, NK cells activity and TNF-a activity in group B were not significantly different from those in group C (P = 0.953, P = 0.130, P = 0.080, respectively) but markedly higher than those in group A (P < 0.05). The results also showed that SPEFs could not only kill tumor cells but also induce antitumor immune response and improve the immune function of the host efficiently.