| 大鼠肾下腹主动脉缺血再灌注对心肌肌浆网钙泵活性及其基因表达的影响 |
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| 引用本文： | 于影,胡杰,高琴,汪思应. 大鼠肾下腹主动脉缺血再灌注对心肌肌浆网钙泵活性及其基因表达的影响[J]. 蚌埠医学院学报, 2010, 35(2): 112-114. DOI: 10.3969/j.issn.1000-2200.2010.02.002 |
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| 作者姓名： | 于影 胡杰 高琴 汪思应 |
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| 作者单位： | 安徽医科大学,病理生理学教研室,安徽,合肥,230032;蚌埠医学院,生理学教研室,安徽,蚌埠,233030;蚌埠医学院,生理学教研室,安徽,蚌埠,233030;安徽医科大学,病理生理学教研室,安徽,合肥,230032 |
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| 基金项目： | 安徽省高等学校省级自然科学研究资助项目(KJ2007B018) |
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| 摘 要： | 目的：观察大鼠肾下腹主动脉缺血再灌注对心肌肌浆网钙泵（sarcoplasmic reticulum Ca^2＋ -ATPase，SERCA）的活性及其基因表达的影响。方法：复制大鼠肾下腹主动脉缺血再灌注（ischemia and reperfusion，I／R）模型。将SD雄性大鼠随机分为假手术组，缺血30min分别再灌注2、12、24和48h（I／R2h组、I／R12h组、I／R24h组、I／R48h组）。采用无机磷比色法检测缺血再灌注不同时间点心肌肌浆网SERCA活性，反转录酶PCR技术测定SERCA基因的表达。结果：I／R24h组、I／R48h组SERCA的活性及基因表达均冠著低于假手术组（P〈0．01）；I／R12h组SERCA的活性均低于似手术组（P〈0．01），但基因表达无明显变化（P〉0．05）；I／R2h组SERCA的活性及其基因表达与假手术组差异均无统计学意义（P〉0．05）。结论：肾下腹主动脉缺血再灌注过程严重影响SERCA的活性及其基因的表达。
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| 关 键 词： | 心脏/生理学 再灌注损伤 大鼠 心肌 肌浆网钙泵 肾下腹主动脉 |
Effects of activity and expression of sarcoplasmic reticulum Ca2+-ATPase in rat heart in infrarenal abdominal aorta occlusion induced ischemia and reperfusion injury |
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| YU Ying,HU Jie,GAO Qin,WANG Si-ying. Effects of activity and expression of sarcoplasmic reticulum Ca2+-ATPase in rat heart in infrarenal abdominal aorta occlusion induced ischemia and reperfusion injury[J]. Journal of Bengbu Medical College, 2010, 35(2): 112-114. DOI: 10.3969/j.issn.1000-2200.2010.02.002 |
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| Authors: | YU Ying HU Jie GAO Qin WANG Si-ying |
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| Affiliation: | 1.Department of Pathophysiology;Anhui Medical University;Hefei Anhui 230032;2.Department of Physiology;Bengbu Medical College;Bengbu Anhui 233030;China |
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| Abstract: | Objective:To investigate the sarcoplasmic reticulum Ca2+-ATPase(SERCA) activity and mRNA expression in rat heart in infrarenal abdominal aorta occlusion induced ischemia and reperfusion injury(I/R).Methods:Forty male Sprague-Dawley rats were randomized into five groups:pseudo-operation group,I/R2h group,I/R12h group,I/R24h group and I/R48h group.Rats in pseudo-operation group were undergone laparotomy only.Meanwhile,rats in I/R groups were reperfused with different hours (2,12,24 and 48 hours) following by ... |
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| Keywords: | heart/physiology ischemia and reperfusion injury rat heart sarcoplasmic reticulum Ca2+-ATPase infrarenal abdominal aorta |
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