肾茶提取物防治大鼠慢性肾功能衰竭的实验研究

目的研究肾茶提取物对慢性肾功能衰竭(CRF)大鼠肾功能的影响。方法采用腺嘌呤致大鼠慢性肾功能衰竭动物模型,将SD大鼠随机分成5组:1组为正常组、2组为模型组、3组为肾茶提取物高剂量组、4组为肾茶提取物低剂量组、5组为尿毒清对照组。将2～5组实验动物腺嘌呤灌胃,18d后收集1组、2组24h尿液,并眼底采血测24h尿蛋白定量(UTP)、血清尿素氮(BUN)、血肌酐(Cr)、尿酸(UA)、血红蛋白(Hb)、尿微量白蛋白(UMA)及肿瘤坏死因子-α(TNF-α),验证造模成功。19d后3、4组给予肾茶提取物,5组给予尿毒清,1、2组给予生理盐水。49d后收集全部大鼠24h尿液,然后处死大鼠,采血及取肾、称肾重、体重。检测UTP、BUN、Cr、Hb、UMA、UA、TNF-α、Ca、P等指标。HE染色观察肾脏病理结构。结果实验前后肾茶高、低剂量组大鼠的肾功能指标与模型组相比较,均具有显著性,与对照组相比,效果亦显著;明显降低CRF大鼠体内TNF-α,明显抑制肾小球系膜细胞及基质增生,减轻肾小管-间质损害。结论肾茶提取物能够降低CRF大鼠UTP、Cr、BUN、UA、P、UMA、TNF-α水平、升高血中Hb、Ca等水平,从而有效改善CRF大鼠的肾功能及免疫功能。

Preventing and Curing Chronic Renal Failure in Rat with Orthosiphon Extract:An Experimental Study

Objective To study the effects of orthosiphon extract on the kidney of chronic renal failure(CRF) in rat.Methods The study was carry our by using animal modol of rat kidney of chronic renal failure induced by adenine,there are five groups:Control group,CRF group,CRF plus high orthosiphon extract group,CRF plus low orthosiphon extract group,control plus Niaoduqing group.The experimental animals of 2-5 group were perfused with adenine for 18 days,then the serum BUN,Cr,UA,Hb,UMA,TNF-αlevel and the UTP of 24h urine were measured,which confirmed that Rat model of chronic renal failure was achieved.The 3rd and 4th group were treated with orthosiphon extract,the 5th group with Niaoduqing,the 1st and 2nd group with normal saline solution for 30 days.Then 24h urine of all rats was collected,besides,all rats were executed following a blood collecting and a kidney extraction.The weight of kidney and body were measured.The serum BUN,Cr,Hb,UMA,UA,TNF-α,Ca,P level and UTP of 24h urine were also measured.The morphological structure of kidney were observed by HE staining.Results Compared with the CRF group and control plus Niaoduqing group,the functional parameters of kidney were significantly different in the treated groups.TNF-α of treated groups decreased significantly in chronic renal failure(CRF) rat,significantly inhibiting the proliferation of renal intercapillary cells and matrix and attenuating the injury of the renal tubule and interstitium.Conclusion Orthosiphon extract can decrease the level of UTP,Cr,BUN,UA,UMA,TNF-α,P and increase the level of Hb,Ca in CRF rat.It can improve effectively the renal function and immunological function.