AQP1在骨巨细胞瘤中的表达和意义

目的研究水通道蛋白1（aquaporin 1,AQP1）在骨巨细胞瘤中的分布及在骨巨细胞瘤组和对照组中的表达差别,并确定AQP1在骨巨细胞瘤各级中的表达差别,从而确定AQP1在骨巨细胞瘤中的作用。方法对42例骨巨细胞瘤标本和24例对照组标本,采用免疫组织化学方法,镜下观察AQP1在组织中的分布,应用自动图像分析检测AQP1在两组中表达情况。结果a）本免疫组化染色显示：AQP1表达于所有标本的肿瘤微血管和小血管内皮细胞上;AQP1表达于大多数骨巨细胞瘤的肿瘤细胞上,AQP1在对照组中无表达或表达很微弱;b）自动图像分析系统所得平均光密度在两组中差别显著（P〈0.01）,AQP1在骨巨细胞瘤中高表达;c）AQP1的表达在骨巨细胞瘤级和骨巨细胞瘤级差别显著（P〈0.01）,而AQP1的表达在骨巨细胞瘤其他各级之间无差别（P〉0.05）。结论大多数骨巨细胞瘤的肿瘤细胞高表达AQP1的原因不明;AQP1在骨巨细胞瘤中高表达,预示其在骨巨细胞瘤的生物学行为中的作用;AQP1的表达在骨巨细胞瘤级和骨巨细胞瘤级差别显著,提示AQP1可能有区分骨巨细胞瘤级和骨巨细胞瘤级的作用。

Expression and Distribution of Aquaporin 1 in Giant Cell Tumor of Bone and Its Significance

Objective To study expression level and distribution of aquaporin 1（AQP1）in giant cell tumor of bone （GCT） group and control group,and to detect the expression difference of AQP1 in GCT s grade,then try to find out the significance of AQP1 in GCT. Method AQP 1 was detected by SP immunohistochemical method. The distribution of AQP1 in tissues was observed in high-scope,and the expression level of AQP 1 was detected by auto-image analysis. Results a）This study showed that AQP 1 protein was strongly expressed in the endothelium of microvessels and small vessels in all cases;AQP 1 protein is mainly localized in the most GCT＇s rumor-cells, b）The Mean Density （MD）by auto-image analysis in GCT was also significantly higher than control groups （P〈0.01）,the data indicates that the expression level of AQP 1 in GCT was higher than that in control groups, c）The expression level in grade Ⅰ and grade Ⅲ of GCT was significantly different （P〈0.01）,but the expression level in other grades was not different （P〉0. 05）. Conclusion The AQP 1 expression level in GCT was remarkably stronger ,signify the role of AQP 1 in GCT＇s biological behaviour,moreover the expression level in grade Ⅰ and grade Ⅲ of GCT was significantly different explains AQP 1 may have the role in differenting grade Ⅰ and grade Ⅲ of GCT.