| 随机扩增多态性DNA技术用于青藏高原型鼠疫菌的比较 |
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| 引用本文： | 崔百忠. 随机扩增多态性DNA技术用于青藏高原型鼠疫菌的比较[J]. 中国媒介生物学及控制杂志, 2009, 20(1): 55-56 |
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| 作者姓名： | 崔百忠 |
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| 作者单位： | 青海省地方病预防控制所鼠疫预防控制科,西宁,811602 |
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| 摘 要： | 目的对青海、西藏和甘肃省（自治区）境内不同宿主体内分离的21株青藏高原型鼠疫耶尔森菌（鼠疫菌）的基因进行分析，旨在比较不同宿主鼠疫菌之间的关系。方法采用随机扩增多态性DNA（RAPD）技术。结果扩增产物在凝胶电泳上显示的条带，其中16007、28001、01080号菌株相同，且与其余18株菌株存在差别。结论该实验中不同宿主体内检出的21株青藏高原型鼠疫菌在遗传学上属于同源。
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| 关 键 词： | 随机扩增多态性DNA 宿主 青藏高原型鼠疫菌 |
Comparison of Qinghai-Tibetau plateau strain of Yersinia pestis from different reservoirs by random amplified polymorphism DNA |
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| CUI Bai-zhong. Comparison of Qinghai-Tibetau plateau strain of Yersinia pestis from different reservoirs by random amplified polymorphism DNA[J]. Chinese Journal of Vector Biology and Control, 2009, 20(1): 55-56 |
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| Authors: | CUI Bai-zhong |
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| Affiliation: | DNA CUI Bai- zhong. (Institute for Endemic Disease Prevention and Control of Qinghai Province, Xining, Qinghai 811602, China) |
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| Abstract: | Objective To study the gene of 21 Qinghai-Tibetau plateau strains of Yersinia pestis isolated from the different reservoirs in Qinghai, Tibet and Gansu, and to know the relationship of these strains from the different reservoir. Methods Random amplified polymorphism DNA （RAPD） was used in this study. Results The electro-phoretic band of amplified products from 16007, 28001 and 01080 Y.pestis strains were similar, but there were difference between three strains and other sample strains. Conclusion 21 strains isolated from the different reservoir were homologous in genetics. |
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| Keywords: | Random amplified polymorphism DNA Reservoir Qinghai-Tibetau plateau strain of Yersinia pestis |
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