新型pH敏感性材料及其给药系统在现代药剂学中的应用进展

 目的 探讨血管内皮生长因子（VEGF）在结直肠癌组织中的表达及其作为肿瘤新生血管标志的临床价值。 方法 采用免疫人VEGF单克隆抗体及鼠抗人因子Ⅷ相关抗原（F-8 RAg）单克隆抗体，通过免疫组化技术对48例结直肠癌手术切除的癌组织及癌旁正常组织（对照组织）中VEGF和F-8 RAg的表达进行检测。 结果 结直肠癌组织VEGF表达阳性率为62.5%（30/48），对照组为16.7%（8/48），χ²＝64.352，P<0.01。结直肠癌组织F-8 RAg标记的MVD为100.9 ± 16.0，对照组为46.8 ± 11.9，t =18.351，P<0.01。VEGF的表达与大肠癌的淋巴结转移（P<0.01）、远处转移（P<0.05）及临床分期有关（P=0.01）；而F-8 RAg标记的MVD与大肠癌的淋巴结转移、远处转移及临床分期无明显相关（P>0.05）。VEGF在结肠癌组织的表达与F-8 RAg的表达无相关性（P>0.05）。 结论 VEGF在结直肠癌组织新生血管有良好表达，可以作为结直肠癌新生血管有价值的标志

The clinical value of Vascular endothelial growth factor as a marker in angiogenesis of colorectal cancer

Objective To determine the expression of vascular endothelial growth factor (VEGF) in colorectal cancer and its clinical value as a marker of neoplasm-related angiogenesis. Methods Samples of colorecatal cancer and adjacent normal (control) tissues were obtained from 48 patients with colorectal cancer, and stained with anti-VEGF monocolonal antibody (mAb) or anti-factor VIII-related antigen mAb (anti-FVIII-RAg mAb). The expression of VEGF and FVIII-RAg in the samples was detected by immunohistochemistry. Results The positive rate of VEGF in colorectal cancer tissue was 62.5% (30/48), significantly higher than that of control tissue 16.7% (8/48), χ²=64.352, P<0.01]. The MVD of colorectal cancer with F-8 RAg marker (100.9 ± 16.0) was significantly greater than that of control group (46.8 ± 11.9, t =18.351, P<0.01). The expression of VEGF was significantly correlated with lymph node metastasis (P<0.01), distant metastasis (P<0.05) and Dukes stages (P=0.01); but MVD had no significant correlation with them (P>0.05). There was no significant correlation between the expression of VEGF and F-8 RAg in colorectal cancer tissue (P>0.05). Conclusions VEGF is highly expressed in the vascular endothelium of neoplasm-related microvessels of colorecatal cancer. It can be used as a marker of angiogenesis in colorecatal cancer.