梅毒螺旋体抗体三种检测方法的价值比较

目的比较三种血清梅毒螺旋体抗体(TPHA)检测方法,优选一种简便、准确的TPHA检测方法。方法采用临床上常用的胶体金法、酶联免疫吸附试验法(ELISA法)、化学发光微粒子免疫分析法(CMIA法)对400例标本进行TPHA检测,分别计算ELISA法、CMIA法、胶体金法检测结果的阳性率、灵敏度和特异度。结果 CMIA法检测TPHA的阳性率、灵敏度和特异度分别为74.50%、99.67%和100%;胶体金法的阳性率、灵敏度和特异度分别为41.25%、55.18%和100%;ELISA法的阳性率、灵敏度和特异度分别为60.25%、80.60%和93.46%。CMIA法检测阳性率和敏感性明显高于ELISA法和胶体金法(P<0.05),而特异性高于ELISA法(P<0.05)。结论CMIA法检测TPHA特异性强、灵敏度高,具有很高的临床应用价值。

Value comparison of three methods for the detection for Treponema pallidum antibody

Objective To compare the three serological methods for Treponema pallidum haemagglutination assay(TPHA) and to select a simple,accurate method for TPHA.Methods Use colloidal gold method,enzyme linked immunosorbent assay method(ELISA method) and chemiluminescent microparticle immunoassay(CMIA) to detect syphilis antibody in 400 cases of specimens,respectively calculated the positive rate,sensitivity and specificity of those three methods.Results The positive rate,specificity and sensitivity for CMIA were 74.5%,99.67% and 100%;for immunoassay of colloidal gold were 41.25%,55.18%,100%;for ELISA were 60.25%,80.60%,93.46%.The positive rate and sensitivity detected by CMIA was significantly higher than by ELISA and colloidal gold method(P<0.05),while the specificity was higher than ELISA(P<0.05).Conclusion CMIA is suitable for TPHA with good specificity and sensitivity and high clinical application value.