微泡增强低频超声空化抑制裸鼠前列腺癌移植瘤

目的观察低频超声（20kHz）辐照联合静脉注射微泡造影剂SonoVue对裸鼠前列腺癌（Dul45）移植瘤的抑瘤效应，并探讨其可能的抑瘤机制。方法通过细胞移植和瘤块移植方法建立24只裸鼠前列腺癌Dul45移植瘤模型，随机分为超声微泡组、单纯超声组、单纯微泡组和对照组，每组各6只。超声微泡组：尾静脉注射0．2mLSonoVue的同时对瘤体行20kHz超声辐照，辐照强度200mW／cm2；单纯超声组：尾静脉注射生理盐水0．2mL，同时超声辐照2min；单纯微泡组：尾静脉注射SonoVue0．2mL同时行假照，各组均隔天1次，共3次，对照组不做任何处理。治疗后测量瘤体大小，绘制瘤体生长曲线，计算抑瘤率。首次治疗后14d剥离瘤体，通过光学显微镜、电子显微镜观察肿瘤组织病理改变。免疫组织化学方法观察CD34阳性染色血管，计算肿瘤微血管密度（microvesseldensity，MVD），比较各组间MVD的差异。结果24只裸鼠均成功植瘤。治疗后超声微泡组瘤体体积均数明显小于其他3组（P〈O．05），抑瘤率为62．7％。光学显微镜下超声微泡组瘤体组织大部分损伤坏死，电子显微镜下超声微泡组肿瘤内微血管的内皮细胞损伤，线粒体肿大，基底膜断裂。超声微泡组瘤体内CD34阳性染色微血管数减少，其MVD值显著低于其他各组。结论20kHz低频超声辐照联合微泡造影剂SonoVue可有效抑制裸鼠人前列腺癌移植瘤的生长，其抑瘤机制可能是通过超声空化效应破坏肿瘤的微血管实现的。

An Experimental Study on Anti-tumor Effect of Microbubble-enhanced Low-frequency Ultrasound Cavitation on Prostate Carcinoma Xenograft in Nude Mice

Objective To investigate the anti-tumor effect induced by low-frequency ultrasound(20 kHz) radiation combined with intravenous injection of microbubbles on human prostate carcinoma xenograft in nude mice,and to discuss its probable mechanism.Methods Human prostate carcinoma xenograft model in 24 nude mice were established with human prostate carcinoma Du145 cells inoculation and sub-graft through mice,which were randomly divided into ultrasound+microbubble,ultrasound,microbubble,and control group,with 6 mic...