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| TS-Fe3O4介导丙型肝炎病毒多表位基因疫苗的研究 | |
| 引用本文： | Yang Y,Kuang Y,Yuan D,Cao LL,Wang BN,Li MY,Zhang ZW. TS-Fe3O4介导丙型肝炎病毒多表位基因疫苗的研究[J]. 四川大学学报(医学版), 2011, 42(6): 757-61, 788 |
| 作者姓名： | Yang Y Kuang Y Yuan D Cao LL Wang BN Li MY Zhang ZW |
| 作者单位： | 1. 四川大学华西基础医学与法医学院微生物学教研室,成都,610041 2. 四川大学生物材料工程研究中心,成都,610064 3. 成都大学医护学院,成都,610015 4. 四川大学华西医院重症监护室,成都,610041 |
| 基金项目： | 国家自然科学基金(批准号30901270)资助 |
| 摘 要： | 目的 研发能诱导有效免疫应答的丙型肝炎病毒(HCV)基因疫苗候选者及探索磁性纳米材料作为基因载体的应用前景.方法 选择5个HCV保守性T/B细胞识别的抗原表位基因,克隆入pcDNA3.1(+),鉴定重组质粒,测定其细胞水平的表达以及在小鼠体内诱导的免疫反应.同时合成壳聚糖(CTS)修饰的Fe3O4磁性纳米微粒(CT... |
| 关 键 词： | 丙型肝炎病毒 多表位基因 磁性纳米材料 壳聚糖 基因投递系统 |
Investigation of HCV multi-epitope gene vaccine loaded by CTS-Fe3O4 |
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| Yang Yuan,Kuang Yu,Yuan Dun,Cao Li-li,Wang Bao-ning,Li Ming-yuan,Zhang Zhong-wei. Investigation of HCV multi-epitope gene vaccine loaded by CTS-Fe3O4[J]. Journal of Sichuan University. Medical science edition, 2011, 42(6): 757-61, 788 | |
| Authors: | Yang Yuan Kuang Yu Yuan Dun Cao Li-li Wang Bao-ning Li Ming-yuan Zhang Zhong-wei |
| Affiliation: | Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. |
| Abstract: | Objective In order to develop a promising HCV gene vaccine candidate to induce effective immune response and explore the application of magnetic nanoparticles as gene delivery system.Methods The DNA fragment containing multi-epitope antigen gene of HCV with five conserved mimotopes was synthesized and cloned into plasmid pcDNA3.1(+).The Fe3O4 modified with chitoson was prepared and the cytotoxicity of the magnetic material was detected in vitro.Analysis of recombinant plasmid in vitro expression,and its imm... |
| Keywords: | HCV Multi-epitope gene Magnetic nanoparticles Chitoson Gene delivery system |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |