| 4重荧光PCR技术在霍乱监测与菌株鉴定的应用研究 |
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| 引用本文： | 黄仕杰,李莉,温慧欣,黄建炜. 4重荧光PCR技术在霍乱监测与菌株鉴定的应用研究[J]. 海峡预防医学杂志, 2011, 17(5): 1-2,65 |
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| 作者姓名： | 黄仕杰 李莉 温慧欣 黄建炜 |
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| 作者单位： | 厦门市疾病预防控制中心,福建,361021 |
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| 基金项目： | 卫生部-福建省医教联合攻关项目(No.WKJ2008-2-39); 厦门卫生局科技计划项目(No.WSK05) |
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| 摘 要： | 目的 应用4重荧光PCR技术检测霍乱弧菌并对菌株进行鉴定.方法 用PCR法对2008-2010年监测的水样、水产品标本及食物中毒疑似菌株以进行检测.同时用细菌学方法分离菌株.再进行PCR检测.结果 1606份标本中.PCR法检出O1群霍乱弧菌阳性标本188份.从中分离到菌株150份(79.8％).O139群霍乱弧菌的P...
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| 关 键 词： | 霍乱弧菌 荧光PCR技术 快速筛检 菌株鉴定 |
Application study of quadruplex real-time PCR surveillance and the strain identification for Vibrio cholerae |
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| HUANG Shi-jie,LI Li,WEN Hui-xin,et al.. Application study of quadruplex real-time PCR surveillance and the strain identification for Vibrio cholerae[J]. Strait Journal of Preventive Medicine, 2011, 17(5): 1-2,65 |
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| Authors: | HUANG Shi-jie LI Li WEN Hui-xin et al. |
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| Affiliation: | HUANG Shi-jie,LI Li,WEN Hui-xin,et al. Xiamen Center for Disease Control and Prevention,Fujian 361021,China |
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| Abstract: | ObjectiveTo detect Vibrio cholerae by quadruplex real-time PCR assay and identify the strains.MethodsThe V.choleraewere detected from 2008 to 2010 collected water,aquatic products and food-poisoning case samples by quadruplex real-time PCR assay and the stains were isolated and parallel study with classical bacteriological method to isolate the strains.And the isolated strains were screened and identified with quadruplex real-time PCR assay.ResultsTotally 188 in 1 606 samples were positive for O1 signal in ... |
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| Keywords: | Vibrio cholerae Real-Time PCR Fast Screening Strain Identification |
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