| OPN反义基因对乳腺癌MDA-MB-231细胞迁移能力的影响 |
| |
| 引用本文： | 姜伟栋,印芳颖,宫路路,张厚忠,乔士兴. OPN反义基因对乳腺癌MDA-MB-231细胞迁移能力的影响[J]. 中国妇幼保健, 2010, 25(4) |
| |
| 作者姓名： | 姜伟栋 印芳颖 宫路路 张厚忠 乔士兴 |
| |
| 作者单位： | 1. 吉林大学第二医院基本外科,吉林,长春,130021
2. 吉林大学中日联谊医院儿科 |
| |
| 摘 要： | 目的:探讨OPN反义基因(ANOPN)对人乳腺癌细胞系MDA-MB-231细胞生长及迁移能力的影响。方法:构建人骨桥蛋白反义基因真核表达质粒pcDNA3.1-ANOPN,将质粒pcDNA3.1-ANOPN、空载体pcDNA3.1(+)和等量的脂质体分别转染人乳腺癌细胞系MDA-MB-231,将转染细胞分别命名为MDA-ANOPN、MDA-vect和MDA,在体外观察各组细胞的迁移能力。结果:MDA-MB-231细胞比MDA-vect及MDA细胞迁移能力弱(P<0.01)。结论:ANOPN可抑制人乳腺癌细胞系MDA-MB-231细胞的迁移能力。
|
| 关 键 词： | 骨桥蛋白 反义基因 乳腺癌 迁移能力 |
Effect of anti-sense gene of osteopontin on migration ability of MDA-MB-231 cells of breast cancer |
| |
| JIANG Wei-Dong,YIN Fang-Ying,GONG Lu-Lu et al. Effect of anti-sense gene of osteopontin on migration ability of MDA-MB-231 cells of breast cancer[J]. Maternal and Child Health Care of China, 2010, 25(4) |
| |
| Authors: | JIANG Wei-Dong YIN Fang-Ying GONG Lu-Lu et al |
| |
| Affiliation: | JIANG Wei-Dong,YIN Fang-Ying,GONG Lu-Lu et al.Department of General Surgery,the Second Hospital of Jilin University,Changchun 130021,Jilin,China |
| |
| Abstract: | Objective:To explore the effect of anti-sense gene of osteopontin(ANOPN) on migration ability of MDA-MB-231 cells of breast cancer.Methods:Eukaryotic expression vector pcDNA3.1-ANOPN was constructed,then pcDNA3.1-ANOPN,empty vector pcDNA3.1(+) and commensurable liposome were used to transfect human breast cancer cell line MDA-MB-321 cells,and the transfected cells were named as MDA-ANOPN,MDA-vect and MDA,respectively.The migration ability of these cells were observed.Results:The migration ability of MDA-MB-... |
| |
| Keywords: | Osteopontin Anti-sense gene Breast cancer Migration ability |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
