| PA-MSHA疫苗增强急性髓细胞白血病源DC对Treg的抑制作用 |
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| 引用本文： | 刘媛媛,张连生,柴 晔,曾鹏云,岳玲玲,吴重阳,李莉娟. PA-MSHA疫苗增强急性髓细胞白血病源DC对Treg的抑制作用[J]. 中国肿瘤生物治疗杂志, 2010, 17(4): 429-433. DOI: 10.3872/j.issn.1007-385X.2010.04.013 |
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| 作者姓名： | 刘媛媛 张连生 柴 晔 曾鹏云 岳玲玲 吴重阳 李莉娟 |
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| 作者单位： | 兰州大学附属第二医院血液肿瘤科,甘肃兰州,730030 |
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| 基金项目： | 甘肃省科技支撑计划项目(No.0804NKCA115) |
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| 摘 要： | 目的： 探讨带有甘露糖敏感血凝菌毛的铜绿假单胞菌（Pseudomonas aeruginosa with mannose sensitive hemagglutination pili，PA-MSHA）疫苗处理后急性髓细胞白血病源性树突状细胞（dendritic cells derived from acute myeloid leukemia，AML-DC）对调节性T细胞（regulatory T cell，Treg）的抑制作用。 方法： rhGM-CSF和rhII-4诱导的AML-DC分为对照组、PA-MSHA组和TNF-α组，培养24 h后观察3组AML-DC的形态、流式细胞术检测各组AML-DC的表型、MTT法和混合淋巴细胞反应检测AML-DC对淋巴细胞增殖的作用。磁珠法分离健康人外周血CD4+T细胞，加入各组AML-DC中诱导Treg，ELISA法检测各组Treg上清液中IL-10、TGF-β的水平，流式细胞术检测Treg表面CD4、CD25的表达，RT-PCR法检测Treg中Foxp3 mRNA的表达水平。 结果： PA-MSHA组和TNF-α组AML-DC呈树突状形态，且CDla、CD80、CD83、CD86和 HLA-DR表达较对照组明显升高（P<0.05）。PA-MSHA组和TNF-α组AML-DC诱导的T细胞增殖能力显著增强（P<0.05）。PA-MSHA组和TNF-α组AML-DC诱导产生的Treg分泌较低水平的IL-10、TGF-β（P<0.05），CD4、CD25的表达及Foxp3 mRNA水平均较对照组明显降低（P<0.05）。上述各指标PA-MSHA组和TNF-α组间均无明显差异。 结论： PA-MSHA疫苗可促进AML-DC的成熟，抑制初始T细胞向Treg的分化，增强AML-DC对AML患者Treg的抑制作用。
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| 关 键 词： | 急性髓细胞白血病；带有甘露糖敏感血凝菌毛的铜绿假单胞菌；树突状细胞；调节性T细胞 |
| 收稿时间： | 2010-04-26 |
| 修稿时间： | 2010-06-05 |
PA-MSHA vaccine enhances inhibitory effect of acute myeloid leukemia-derived dendritic cells on regulatory T cells |
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| LIU Yuan-yuan,ZHANG Lian-sheng,CHAI Ye,ZENG Peng-yun,YUE Ling-ling,WU Chong-yang and LI Li-juan. PA-MSHA vaccine enhances inhibitory effect of acute myeloid leukemia-derived dendritic cells on regulatory T cells[J]. Chinses Journal of Cancer Biotherapy, 2010, 17(4): 429-433. DOI: 10.3872/j.issn.1007-385X.2010.04.013 |
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| Authors: | LIU Yuan-yuan ZHANG Lian-sheng CHAI Ye ZENG Peng-yun YUE Ling-ling WU Chong-yang LI Li-juan |
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| Affiliation: | Department of Hematology and Oncology, Second Hospital Affiliated to Lanzhou University, Lanzhou 730030, Gansu, China;Department of Hematology and Oncology, Second Hospital Affiliated to Lanzhou University, Lanzhou 730030, Gansu, China;Department of Hematology and Oncology, Second Hospital Affiliated to Lanzhou University, Lanzhou 730030, Gansu, China;Department of Hematology and Oncology, Second Hospital Affiliated to Lanzhou University, Lanzhou 730030, Gansu, China;Department of Hematology and Oncology, Second Hospital Affiliated to Lanzhou University, Lanzhou 730030, Gansu, China;Department of Hematology and Oncology, Second Hospital Affiliated to Lanzhou University, Lanzhou 730030, Gansu, China;Department of Hematology and Oncology, Second Hospital Affiliated to Lanzhou University, Lanzhou 730030, Gansu, China |
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