TNF-α在体外具有促进猪卵泡内膜细胞增殖的作用 |
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引用本文: | 洪岭,滕晓明,李昆明,龚瑶,张燕,杨芳,张博,童晓文. TNF-α在体外具有促进猪卵泡内膜细胞增殖的作用[J]. 华西医学, 2010, 0(1): 46-48 |
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作者姓名: | 洪岭 滕晓明 李昆明 龚瑶 张燕 杨芳 张博 童晓文 |
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作者单位: | [1]同济大学附属第一妇婴保健院,上海200040 [2]同济大学附属同济医院妇产科,上海200065 |
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摘 要: | 目的:研究肿瘤坏死因子(TNF-α)对猪卵泡内膜细胞增殖情况的影响,探讨TNF-α在多囊卵巢综合征(P-COS)发病机制中的作用。方法:在体外培养的猪卵泡内膜细胞培养液中加入TNF-α(10、100和1000 pg/mL),采用CFDA SE细胞增殖检测试剂盒和流式细胞仪观察TNF-α对卵泡内膜细胞增殖情况的影响。结果:72 h后TNF-α(100和1000 pg/mL)组卵泡内膜细胞平均荧光强度(MFI)明显低于对照组,有统计学差异。结论:TNF-α具有促进卵泡膜细胞增殖的作用,这个现象可能与PCOS患者发生卵巢白膜增厚、卵泡膜细胞增生病变有关。
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关 键 词: | TNF-α PCOS CFDA SE 卵泡内膜细胞 |
TNF-alpha can Promote the Proliferation of Porcine Theca Interna Cells in Vitro |
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Affiliation: | HONG Ling,TENG Xiao-ming,LI Kun-ming,et al.(The First Maternal and Infant Health Care Hospital,Tongji University,Shanghai 200040,China ) |
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Abstract: | Objective: To study the effect of tumor necrosis factor-alpha(TNF-alpha) on the proliferation of theca interna cells(TI) and to explore the role of TNF-alpha in the polycystic ovary syndrome pathogenesis.Methods: Different concentrations of TNF-αlpha(10 pg/mL,100 pg/mL,1000 pg/mL) were treated with TI.Proliferation of TI were detected by CFDA SE kit and Flow Cytometry.Results: After 72 h,The mean fluorescence intensity(MFI) in TI with TNF-alpha 100 pg/mL and 1000 pg/mL were lower obviously than controls(P〈0.05).Conclusion: TNF-αlpha can promote the proliferation of theca interna cells,which may explain the phenomenon: PCOS usually accompanies thick ovarian tunica albuginea and proliferative theca cells. |
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Keywords: | TNF-α PCOS CFDA SE theca interna cells |
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