Adherence of Staphylococcus aureus isolated in peritoneal dialysis-related exit-site infections to HEp-2 cells and silicone peritoneal catheter materials |
| |
Authors: | Kreft B; Ilic S; Ziebuhr W; Kahl A; Frei U; Sack K; Trautmann M |
| |
Institution: | Medizinische Klinik I, Medizinische Universitat Lubeck, Lubeck, Germany; Institut fur Molekulare Infektionsbiologie, Universitat Wurzburg, Wurzburg, Germany; Abteilung Nephrologie, Virchow Klinikum, Berlin, Germany; Institut fur Mikrobiologie und Hygiene, Universitat Ulm, Ulm, Germany; Corresponding author address: Department of Internal Medicine I, Division of Nephrology, Medical University of Lubeck, Ratzeburger Allee 160, 23538 Lubeck, Germany |
| |
Abstract: | Background. Peritoneal catheter exit-site infections
cause a relevant morbidity in peritoneal dialysis patients and are
frequently caused by Staphylococcus aureus. We tested
the hypothesis that adherence of exit-site-derived
S.aureus to epithelial cells and peritoneal catheter
silicone tubes discriminates virulent and less virulent strains.
Methods. The binding of isolated S.
aureus to an epithelial cell line (HEp-2) and to silicone tubes
was analyzed using light-microscopy or radioactive labeling of bacteria.
Results. Of 378 exit-site swabs, 99% (26%) were
positive for microbial growth. S. aureus was cultured
in 25 of 99 positive swabs; three of 13 swabs taken in exit-site infections
grade 3 and 4 that had tested positive for S. aureus.
Adherence of S. aureus from exit-site infections grade
2, 3 and 4 to Hep-2 cells did not differ from adherence of bacteria
isolated from asymptomatic or moderately inflamed catheter exit sites
(grade 0-2). However, binding of S. aureus to silicone
tubes was enhanced in grade 0/1 compared with grade 2-4 exit-site isolates.
Conclusions. Staphylococcus
aureus is an important pathogen in CAPD-related exit-site
infection being isolated in about 6.6% of all exit-site swabs (and in 25%
of all positive swabs). Silicone-adhesive strains may be of more clinical
significance in peritoneal dialysis patients since adhesion to silicone was
increased in S. aureus strains isolated in more severe
exit-site infections. |
| |
Keywords: | |
本文献已被 Oxford 等数据库收录! |
|