应力刺激对于后纵韧带骨化因子的影响

目的]建立颈椎后路手术动物模型，增加后纵韧带的张力，检测后纵韧带中BMP-2及BMP-7的表达及水平，探讨机械应力刺激对后纵韧带骨化的作用机理。方法]S-D大鼠100只，分为5组，每组20只，手术方式：（1）颈椎后路全椎板切除组；（2）颈椎后路全椎板切除＋肌肉切断组；（3）单纯椎旁肌止点切断组；（4）假手术组，仅作切口，剥离椎旁肌暴露；（5）对照组，不作任何处理。分别于术后1、2、4、8周，取实验动物手术节段后纵韧带标本，PCR方法检测BMP-2及BMP-7的表达水平。结果]（1）颈椎手术后，全椎板切除＋肌肉切断组BMP-2，BMP-7水平上升最明显，与对照组比较有统计学差异；（2）颈椎后路全椎板切除组BMP-2，BMP-7水平水平上升，与对照组比较有统计学差异；（3）单纯椎旁肌止点切断组BMP@，BMP-7水平上升，与对照组比较有统计学差异；（4）假手术组BMP-2，BMP-7水平与对照组比较无明显变化。结论]手术导致的机械刺激提高BMP-2，BMP-7在大鼠后纵韧带内的表达水平；认为虽然动物模型不能完全模拟人体情况，但是BMP-2，BMP-7在OPLL的早期可能具有始动作用，而BMP-7在后续的骨化过程中可能继续发挥作用。建议临床手术时应尽量采取微创，减少术后不稳增加后纵韧带的应力。

Influence of different operations on BMP-2, BMP-7 in rat posterior longitudinal ligament

Objective]To discuss the initiating and developing mechanism of OPLL,and the influence of different operations on happening and developing of OPLL.Method]We subgroup healthy adult S-D rats into groups,did operation on C 3~6 level.The first group:laminectomy;the second group:laminectomy and muscle cutting;the third group:muscle cutting only;the fourth group:exposure only;contrasting group:no treatment.We got the tissue of posterior longitudinal,ligament at 1,2,4,and 8 weeks post-operation and quantify BMP-2 and BMP-7 by PCR method.Result]BMP-2,BMP-7 increased significantly in the 1st,2nd and 3rd group compared with the contrastinggroup;BMP-2,BMP-7 of only exposure group had no significant difference compared with the contrasting group.Conclusion]BMP-2 and BMP-7 play important roles in the early stage of OPLL,they could be the on-setting factors of OPLL.BMP-7 may play an important roles in the later ossification period.