Mdm2基因扩增在贲门癌中的作用及临床病理意义

目的 研究Mdm2基因扩增与贲门癌的关系及其与各种临床病理因素之间的相关性。方法 取新鲜贲门癌组织，用酚氯仿异物醇法提取基因组DNA；用差异聚合酶链反应（differential polymerase chain reaction,dPCR)技术检测mdm2基因扩谱。结果 38例贲门癌组织中7例有mdm2基因扩增（18．2％），高中分化组的mdm2基因扩增阳性率（33．3％）较低分化组（5．0％）高（P＜0．05）；而mdm2基因扩增与其它临床病理因素之间，如年龄、性别、组织类型和淋巴结转移等均无相关性（P＞0．05）。结论 mdm2基因扩增在贲门癌并非少见，提示mdm2基因扩增可能是导致贲门癌野生型p53功能丧失的重要遗传改变；检测贲门癌mdm2基因扩增对估计该肿瘤的预后可能有所帮助。

AMPLIFICATION OF GENE MDM2 IN CARDIA GASTRIC CARCINOMA AND ITS CLINICOPATHOLOGICAL IMPLICATION

Objective　To determine the prevalence and potential clinical value of mdm2 amplification in cardia gastric carcinoma.Methods　Mdm2 amplification was examined by differential polymerase chain reaction (dPCR).Results　Mdm2 amplification was found in 7 of 38 cases (18.2%). Otherwise, the positive rate of mdm2 gene amplification in higher grade group was higher than that in the lower grade group (33.3% vs 5%), and the difference was significant (P<0.05). There was no correlation observed between the alteration and other clinicopathological parameters, such as sex, age, histological subtype, lymph node metastasis,et al. Conclusion　The data suggest that mdm2 amplification might play an important role in cardia gastric carcinoma. It maybe a mechanism for cells to escape from the regulatory pathway of p53. The detection of the alteration may be helpful to assess the prognosis of cardia gastric carcinoma.