非平衡法在乙型肝炎e抗体和核心抗体检测中的效果评价

目的 探讨酶联免疫吸附试验非平衡法对乙型肝炎e抗体和核心抗体检测结果的影响.方法 收集电化学发光免疫分析法（ECLIA）检测抗-HBe阳性标本44例和抗-HBc阳性标本57例,再用酶联免疫吸附试验法（ELISA）对阳性标本进行检测,样本加入反应孔中分别于0min、30min后加入酶标志物,余下步骤均严格按照说明书操作.结果 对ECLIA检测抗-HBe阳性标本44例、抗-HBc阳性标本57例进行ELISA复检后,平衡法抗-HBe的漏检率为52.3％,抗-HBc漏检率为40.4％;非平衡法抗-HBe的漏检率为34.1％,抗-HBc漏检率为24.6％.结论 竞争法检测抗-HBe和抗-HBc时应适当延迟加酶的时间,以降低漏检率.

Evaluation of Non Equilibrium Method in the Detection of e Antibody and Core Antibody in Hepatitis B

Objective To explore the influence of enzyme- linked immunosorbent assay method of non- equilibrium on the detection of e antibody and core antibody in hepatitis B. Methods 44 cases of anti-HBe positive and 57 eases of anti-HBc positive specimens determined by electrochemical luminescence immunoassay were analyzed by enzyme linked immunosorbent again. The enzyme marker were added into reaction holes at 0 min, 30 min after samples add respectively, the remaining steps were followed in strict accordance with the manual operation. Results The miss positive detection rate of anti-HBe and anti-HBc analyzed by equilibrium method were 52.3% and 40.4% respectively. The miss positive detection rate of anti-HBe and anti-HBc analyzed by non equilibrium method were 34.1% and 24.6% respectively. Conclusion It would be better to prolong the adding time of enzyme in the detection of anti-HBe and anti-HBc by enzyme-linked immunosorbent assay in order to increase the detection sensitivity.